| 中国庚型肝炎病毒河南株非结构基因（NS）3区cDNA的克隆 … |
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| 引用本文： | 陈刚,谭文杰. 中国庚型肝炎病毒河南株非结构基因（NS）3区cDNA的克隆 …[J]. 中华实验和临床病毒学杂志, 1998, 12(1): 7-11 |
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| 作者姓名： | 陈刚 谭文杰 |
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| 作者单位： | 中国预防医学科学院病毒学研究所，河南省周口市卫生防疫站 |
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| 基金项目： | 国家“863”,“九五”攻关课题 |
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| 摘 要： | 目的 为了研究中国庚型肝炎病毒（ＨＧＶ）非结构（ＮＳ３）区基因结构特征。方法 利用逆转录－半巢式－聚合酶链反应从河南１份ＨＧＶ ＲＮＡ阳性血清获得覆盖ＨＢＶ ＮＳ３全长ｃＤＮＡ的４个片段，并克隆到ｐｃＤＮＡⅡ载体中，采用Ｓａｎｇｅｒ双脱氧末端终止法测定全部ｃＤＮＡ序列。结果 发现克隆到的包括ＨＢＶ ＮＳ３区在内的ｃＤＮＡ序列和度为２１３７个核苷酸，编码７１１个氨基酸。与国内外已测定的５株全序列的相
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| 关 键 词： | 庚型肝炎病毒 cDNA克隆 病毒基因 序列分析 |
Cloning and sequencing of cDNA in NS3 region of Chinese hepatitis G virus isolated from Henan Province |
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| G Chen,W Tan,M Zhan. Cloning and sequencing of cDNA in NS3 region of Chinese hepatitis G virus isolated from Henan Province[J]. Chinese journal of experimental and clinical virology, 1998, 12(1): 7-11 |
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| Authors: | G Chen W Tan M Zhan |
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| Affiliation: | Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052. |
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| Abstract: | To study the genetic characteristics of non-structural region 3 of HGV in China, a HGV-RNA positive sample was collected from Henan. HGV RNA was extracted and amplified by RT-nested-PCR. We got four overlap fragments and they were cloned into plasmid vector pcDNA II. The cDNA was sequenced by the Sanger's method. Nucleotide homology between Henan strain and other reported HGV isolates (PNF2161, R10291, GBV-C) was 85.7% to 93.8% over the 2137 nucleotides examined. Comparison of amino acid sequence deduced from Henan strain with that of other isolates showed 96.1% to 97.7% homology. Three amino acid variations were found in NS3 region of all the three Chinese isolates of HGV. In addition, a N-glycosylation site was found in the NS3 region of all the six isolates. |
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| Keywords: | Hepatitis G virus Cloning of cDNA Gene viral Sequence analysis |
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