N糖基化对SMMC7721移植瘤生长及FAS,NM23,ICAM-1表达的调控

目的观察N糖基化抑制剂swainsonine对人肝癌细胞株SMMC7721体内生长的影响及探讨其可能的作用机制.方法将SMMC7721接种于裸鼠,对比研究饮用含1 mg/Lswainsonine饮用水的荷瘤裸鼠和对照组裸鼠的生存期及肿瘤大小指数,运用免疫组织化学的方法观察两组瘤组织标本的FAS,NM23,ICAM-1的表达情况.结果 swainsonine能延长实验组荷瘤裸鼠的生存期,抑制SMMC7721在荷瘤小鼠上的生长,其肿瘤生长抑制率为36%.FAS,NM23,ICAM-1的表达在实验组明显增强.结论 N糖基化抑制剂swainsonine能抑制人肝癌细胞株SMMC7721在裸鼠体内的生长,其对肿瘤组织fas,nm23 ICAM-1表达的调控可能与其作用机制有关.

Effects of de-N-glycosylation on the growth and expression of FAS,NM23, ICAM-1 in SMMC7721 cell line in mice

AIM To study the effects of de-N-glycosylated swainsonine on the in vivo growth and expression of FAS, NM23, ICAM-1 in SMMC7721 cell line. METHODS Balb/ c nude mice with SMMC7721 xenografts were supplied with drinking water containing swainsonine 1.0mg/L during the experiment. The effects of swainsonine on the tumor cell growth in vivo were determined by measuring the tumor size and survival time of the mice. The expression of NM23, FAS and ICAM-1 in swainsonine treated or untreated murine tumor xenografts was detected by immunohistochemical staining, the gradings of staining patterns were referred to Berry' s grading.RESULTS Swainsonine reduced SMMC7721 growth rates by 36% and prolonged the life of mice with SMMC7721 xenografts. Swainsonine also increased the expression of FAS, NM23 and ICAM-1 in xenografts. CONCLUSION Swainsonine is useful in the treatment of SMMC7721 tumor in vivo, regulation of the expression of nm23, fas and ICAM-1 may be one of its antitumor mechanisms.