| E2F陷阱DNA对前列腺癌细胞增殖的影响 |
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| 引用本文： | 王涛,张鹏举,翟静,张媛英,顾洪雁,张凤珍. E2F陷阱DNA对前列腺癌细胞增殖的影响[J]. 泰山医学院学报, 2005, 26(2): 92-94 |
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| 作者姓名： | 王涛 张鹏举 翟静 张媛英 顾洪雁 张凤珍 |
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| 作者单位： | 1. 泰山医学院生物化学教研室,山东,泰安,271000
2. 山东大学医学院生物化学教研室,山东,济南,250012 |
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| 摘 要： | 目的 观察转录因子E2F陷阱DNA策略对雄激素非依赖性前列腺癌细胞PC-3M增殖的影响。方法 采用脂质体Lipofectarnine将E2F decoy DNA、ARE decoy DNA和Control decoy DNA分别转染PC-3M细胞，倒置相差显微镜观察细胞形态变化，MTT检测其对细胞增生活性的影响。结果 E2F decoy DNA转染后的PC-3M细胞的生长受到明显抑制，转染后的细胞形态变化。结论 E2F decoy DNA明显抑制雄激素非依赖性前列腺癌PG3M细胞增殖。
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| 关 键 词： | 转录因子 E2F 前列腺癌 细胞增殖 |
| 文章编号： | 1004-7115（2005）02-0092-03 |
| 收稿时间： | 2004-12-23 |
| 修稿时间： | 2004-12-23 |
Apoptosis induction by E2F decoy DNA of the prostate cancer cell line PC-3M |
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| WANG Tao,ZHANG Peng-ju,ZHAI Jing,ZHANG Yuan-ying,GU Hong-yan,ZHANG Feng-zhen. Apoptosis induction by E2F decoy DNA of the prostate cancer cell line PC-3M[J]. Journal of Taishan Medical College, 2005, 26(2): 92-94 |
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| Authors: | WANG Tao ZHANG Peng-ju ZHAI Jing ZHANG Yuan-ying GU Hong-yan ZHANG Feng-zhen |
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| Abstract: | Objective: To observe effect of E2F decoy DNA on the proliferation of androgen-independent prostate cancer cell line PC-3M. Methods: E2F decoy DNA, ARE decoy DNA and Control decoy DNA were respectively transfected into PC-3M cells with lipofectamine. The change of cell morphology was observed by inverted phase contrast microscope. Their effecet on cell proliferative activity was detected by MTT assay. Rseults: The PC-3M cell growth was inhibited after transfection. Conclusion: E2F decoy DNA inhibites the proliferation of androgen-independent prostate cancer cell lines PC-3M. |
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