不同产地穿心莲的含量测定、化学指纹图谱及抑菌活性评价

目的：从化学和生物两方面综合考察不同产地穿心莲药材品质差异。方法：采用微量量热法比较13批不同产地穿心莲药材的抗痢疾杆菌生物活性。同时建立穿心莲的UPLC指纹图谱，采用偏最小二乘法（PLS）回归分析抑菌活性与化学成分之间的相关性。结果：13批不同产地的穿心莲药材使痢疾杆菌生长代谢的时间延长，生长速率常数减小，表明不同产地穿心莲对痢疾杆菌的生长代谢均有不同程度的抑制作用，且以西双版纳产地的穿心莲药材抑菌效果最好。此外，通过偏最小二乘法回归分析找到了四个与抑菌活性密切相关的化学成分。结论：不同产地穿心莲药材质量差异较大，云南、海南、广西和四川综合质量较好。生物活性测定结合化学指纹图谱评价药材质量的方法，可准确、可靠地评价不同产地穿心莲药材的质量，为其质量控制提供依据。

Evaluation of Chemical Contents, Fingerprint and Antimicrobial Activity of Andrographis paniculata (Burm. f.) Nees Harvested in Different Regions

Objective: The aim of the study was to evaluate the quality of Andrographis paniculata (Burm. f.) Nees harvested in different regions of China. Method: The inhibitory effects of A. paniculata on Shigella dysenteriae growth were investigated by microcalorimetry. UPLC fingerprints of the A. paniculata samples were acquired and then analyzed the correlation with the antimicrobial activity by partial least squares (PLS) analysis. Result: Compared with the control group, all A. paniculata samples from different regions showed inhibitory effects to S. dysenteriae with extending of growth time and decrease of metabolism. And the samples from Yunnan Xishuangbanna had stronger antimicrobial effects than others. According to PLS analysis, there were four chemical contents correlative to the activity of antimicrobial effect. Conclusion: The harvest region had significant influence to the quality of A. paniculata and Yunnan, Hainan, Sichuang and Guangxi were the good harvest regions. The combined evaluation of biological activity and chemical fingerprint provides ability to integrally evaluate the quality of A. paniculata.