深圳市乙型肝炎病毒基因型分子流行病学研究

目的探讨深圳市宝安区乙型肝炎患者血清中乙肝病毒（HBV）基因型的分布情况，了解各基因型与HBV—DNA水平、HBeAg、肝损程度、抗病毒疗效、疾病进展和预后关系。方法采用s基因片段测序的基因分型法，通过上下游引物经PCR反应体系和操作程序扩增S基因片段，经1．5％琼脂糖凝胶电泳，用3730双向测序技术，将130例HBV—DNA阳性患者测序结果在NCBI上进行HBV基因分型，得出基因型。结果宝安区HBV基因型只有B型和C型，无其他型和混合型，B型占55．74％，C型占44．26％，无性别和年龄分布差异（P〉0．05）。B型与C型HBV—DNA水平无显著性差异（P〉0．05），B基因型HBeAg阳性率较c基因型高，C型肝损程度比B型重，进展为肝硬化、肝细胞性肝癌的几率高，两者相比有显著性差异（P〈0．01）。结论研究病例HBV基因型以B型为主，C型次之。C基因型患者病情重，进展快，预后差，值得引起临床医生重视。

Molecular epidemiology study on hepatitis B virus in Baoan District of Shenzhen City

Aim To explore the distribtition of serum hepatitis B virus genotype in patiens with hepatitis B in Baoan District of Shenzhen. Methods S-gene segments were amplified through upsteam and downsteam primers by PCR reaction system with 1.5% agarose gel electrophoresis ,the DNA fragments of 130 HBV-DNA-positive patients were sequened on 3730XL DNA Analyzers. The genotypie assay was completed. Results There were only two genotypes （type B and type C） in HBV-positive patients in Baoan District. Their percentage of tpye B and type C accounted for 55.74%,44.26% respectively; The distribution had no differences between sex and age（P〉0.05）.. The positive rate of genotype B was higher than that of genotype C. While level damage in genotype C was severer with higher probability of development to cirrhosis and hepatoeellular carcinoma, showing significant diffeences （P〈0.01）. Conclusion In Baoan District genotype B is predominant followed by genotype C. HBV-positive patients of genotype C develop rapidly progress with poor proghosis.