应用套式聚合酶链反应技术诊断人巨细胞病毒宫内感染

用套式聚合酶链反应（ＲＴ－ＰＣＲ）技术，对妊娠早、中期各３０例及妊娠晚期３１例孕妇进行母血、脐血及胎盘人巨细胞病毒（ＨＣＭＶ）ＤＮＡ测定。结果：早期妊娠妇女中，２０例ＨＣＭＶ阳性，其中８例发生宫内传播（绒毛组织中ＨＣＭＶ－ＤＮＡ阳性）；妊娠中期妇女中，２１例ＨＣＭＶ阳性，其中７例发生宫内传播（胎盘、脐血ＨＣＭＶ－ＤＮＡ阳性）；妊娠晚期妇女中，２３例ＨＣＭＶ阳性，其中１８例发生宫内传播。提示：套式Ｐ

Detection of Intrauterine Transmission of Human Cytomegalovirus by Nested Polymerase Chain Reaction

bjective Using nested polymerase chain reaction(PCR)to detect Intrauterine transmission of human cytpmegalovirus(HCMV). Methods Ninety one cases of pregnant women were devided into three groups according to their gestational stages.In the first and second trimesters,they terminated pregnancy voluntarily.HCMV- DNA in maternal and cord blood as well as placenta were detected respectively by nested PCR. Results In the first trimester,20 out of 30 cases were infected,Intrauterine transmission occured in 8 of the 20 infected mothers(HCMV-DNA were found in the chorionic villus).In the second trimester,21 out of 30 cases were infected,7 of them transmitted HCMV to their fetus through placen- ta. In this term,the placenta infection rate is 40%(12/30).In the third trimester,23 out of 31 cases women infected HCMV,18 of them transmitted HCMV to their fetus through placenta,the placenta infection rate is 66. 67%(20/30).The congenital infection rate is 58.06%(18/31),much higher than the reported levels marked by cord sera IgM and(or)infants viremia. Concludions HCMV transmitted from mother to infant mainly through plancenta. Nested PCR provides a valuable method that can detected virus infection not limited by virus repreduction state and human immuno-reaction ability.