p53抑癌基因蛋白在外阴鳞癌和不典型增生组织中的表达

应用免疫组化方法，对外阴鳞癌、外阴白色病变和不典型增生组织中ｐ５３基因蛋白的表达进行检测。并与胸腹壁、腿部及外阴鳞癌旁正常皮肤（正常皮肤）组织进行对照。结果：外阴鳞癌组织中ｐ５３基因蛋白的阳性率为５２．９％（９／１７）；外阴硬化苔癣型营养不良组织中为１３．６％（３／２２）；增生型营养不良组织中为１４．７％（５／３４）；混合型营养不良组织中为２０．８％（１０／４８）；不典型增生组织中为５４．５％（６／１１）。正常皮肤组织中均为阴性。提示：ｐ５３基因蛋白在外阴鳞癌和外阴白色病变的各型营养不良组织中，均有不同程度的过度表达，外阴鳞癌和不典型增生组织中的表达阳性率，显著高于外阴白色病变的各型营养不良组织（Ｐ＜０．０１）。

Expression of p53 Gene Protein in Squamous Cell Carcinoma and Precancerous Lesion of Vulva

bjectives To investigate the relationship between p53 gene protein expression and vulvar carcino- ma. Methods Expression of p5 3 gene mutative protein was determined by immunohistochemical tech- nique in 17 specimens of vulva squamous cell carcinoma,11 of atypical hyperplasia , 22 of lichen sclero- sus et atrophicus, 34 of hyperplastic dystrophy, 48 of mixed dystrophy, Normal skin samples from ab- domen and legs, and adjacent tissues of vulvar cancer were served as controls. Results All specimens of normal skin showed no p53 gene mutative protein expression. However, p53 protein positive rates were 52.9% (9/17) in carcinoma group, 54.5% (6/11) in atypical hyperpla- sia, 13.6% (3/22) in llchen sclerosus et atrophicus group, 20.8% (10/48) in mixed dystrophy, 14.7% (5/34) in hyperplastic dystrophy. Conclusions p53 gene mutative protein had a higher expression rate in vulva squamous cell carci- norna and precancerous lesion than that in various types of vulva dystrophy (P < 0.01). These data sug- gested that overexpression of p53 gene mutative protein may be related to the oncogenesis of vulva ma- lignancy.