华支睾吸虫成虫14～33 ku抗原诊断价值的研究

目的探讨纯化华支睾吸虫成虫14~33 ku抗原在华支睾吸虫病免疫诊断中的应用价值。方法使用SDS-PAGE和电洗提法,从可溶性华支睾吸虫成虫抗原中分离纯化14~33 ku抗原,用此抗原经ELISA方法检测华支睾吸虫病、卫氏并殖吸虫病、日本血吸虫病、姜片虫病患者血清及健康人血清特异性IgG抗体,并与粗制可溶性成虫抗原和可溶性成虫脱脂抗原ELISA结果相比较。结果检测63例华支睾吸虫病患者血清,纯化华支睾吸虫成虫14~33 ku抗原ELISA阳性率为76.2%,可溶性成虫抗原和可溶性成虫脱脂抗原ELISA阳性率均为100%;检测25例卫氏并殖吸虫病、85例日本血吸虫病、27例姜片虫病患者血清,14~33 ku抗原的ELISA交叉反应阳性率分别为8.0%、3.5%、0,而可溶性成虫抗原分别为80.0%、62.4%、14.8%,可溶性成虫脱脂抗原分别为64.0%、55.3%、7.4%;检测127例健康人血清,14~33 ku抗原的ELISA假阳性率为0,可溶性成虫抗原和可溶性成虫脱脂抗原的假阳性率分别为5.5%、3.1%。结论纯化华支睾吸虫成虫14~33 ku抗原用于华支睾吸虫病免疫诊断的特异性优于粗抗原,但敏感性较低。

Evaluation on diagnostic efficacy of 14-33 ku antigens purified from the soluble adult worm antigens of Clonorchis sinensis

Objective Objective To investigate the diagnostic efficacy of purified 14-33 ku antigens in immunodiagnosis of clonorchiasis.Methods The 14-33 ku antigens was purified from the soluble adult worm antigens of clonorchis sinensis by 12% SDS-PAGE and elution,and was used to detect specific IgG antibodies in sera of patients with clonorchiasis,paragonimiasis,schistosomiasis japonica,fasciolopsiasis and healthy persons by ELISA.The soluble adult worm antigen(AWA) and the soluble degrease adult worm antigen(DAWA) were used in the control test.Results The sensitivities were 76.2% by 14-33 ku antigens and 100% by AWA and DAWA for detecting 64 cases of clonorchiasis.The cross reaction rates were 8.0%,3.5%,0 respectively by 14-33 ku antigens for detecting 25 cases of paragonimiasis,85 cases of schistosomiasis japonica and 27 cases of fasciolopsiasis,whereas the cross reaction rates were 80.0%,62.4%,14.8% by AWA and 64.0%,55.3%,7.4% by DAWA respectively.The false positive rates were 0,5.5% and 3.1% respectively by 14-33 ku antigens,AWA,DAWA for detecting 127 cases of healthy persons.Conclusion Conclusion The purified 14-33 ku antigens of clonorchis sinensis has higher specificity and lower sensitivity in detecting the specific IgG of clonorchiasis compared to the soluble raw antigens of clonorchis sinensis.