Allotypic suppression of adult mouse spleen cells

Adult mouse spleen cells were transferred into irradiated adult mice with xenogeneic erythrocytes. Antibody-producing cells were measured by the localized haemolysis in gel (LHG) assay. Anti-allotype serum directed against the γG_2a allotype of the donor cells, given to the recipients at the same time as antigen and spleen cells, suppressed the plaque-forming cells (PFC) producing antibody of that allotype, and had little effect on the other classes investigated.