UTI和丹参对大鼠肝脏再灌注损伤保护作用的实验研究

目的 :研究乌司他丁 ( Urinary Trypsin Inhibitor,简称 UTI)和丹参两种药物对大鼠肝脏再灌注损伤的保护作用 ,为临床肝动脉阻断性手术保护肝脏再灌注损伤提供参考依据。方法 :1采用 2× 2析因设计的双盲实验研究方法 ,并采用空白对照。2检测静脉血中血管内皮素 ( ET)、透明质酸酶 ( HA)、门冬氨酸氨基转移酶 ( AST)、丙氨酸氨基转移酶 ( ALT)和乳酸脱氢酶 ( LDH)五项指标。 3应用 SASv6.1 2统计分析软件进行分析。结果 :各实验组的各项指标与实验对照组比较均有统计学意义 ,P<0 .0 1 ;UTI和丹参两种药物均有减轻大鼠肝脏再灌注后损害的作用 ( P<0 .0 1 )。两药合用虽然其检测数据的均数值都有下降趋势 ,但是 ,除 ET项目外 ,其他项目无统计学意义 ,两药比较无统计学意义。结论 :UTI和丹参两种药物均有减轻大鼠肝脏动脉阻断再灌注后肝功能损害的作用

PROTECTIVE EFFECTS OF UTI AND DANSHEN ON THE LIVER ISCHEMIA-REPERFUSION INJURY IN RATS

Objective:To study the efficacy of the medicines UTI and "DANSHEN"(an extract preparation of herb) in the rat liver ischemia reperfusion.Methods:1.Adopting the factorial design with double blind.2. Testing the blood specimens which included ET,HA,AST and LDH.3. Analyzing the data of ET,HA,AST,ALT and LDH with the software of SAS v6 12.Results:All experimental groups to be compared with experimental control group with statistical significance ( P <0 01).It was shown that UTI and DANSHEN could reduce the damage of liver ischemia reperfusion injury( P <0 01).Besides the data of ET,the two medicines have no coordinate and increasing effect.Conclusion:Both UTI and DANSHEN have protective effect of rat's hepatic ischemia reperfusion injury.