实时荧光定量PCR分析雌激素受体相关受体α,β,γ与雌激素受体α,β在卵巢癌中mRNA的表达模式

目的:明确孤儿受体—雌激素受体相关受体(estrogen receptor-related recep-tor,ERRs)各亚型以及雌激素受体亚型在卵巢癌中mRNA的表达模式。方法:采用Light-Cycler-PCR定量检测40例卵巢癌标本以及15例正常卵巢组织中各种ERRs亚型和ERs亚型mRNA的表达丰度。采用ELISA方法分析各样本血清CA-125水平。结果:卵巢癌组织中ERRαmRNA表达的丰度和阳性表达率显著高于正常卵巢组织(P=0.04和P=0.02),ERRβ在卵巢癌组织和正常卵巢组织中的RNA表达的丰度和阳性表达率差异无统计学意义(P>0.05);ERRγmRNA在正常卵巢以及卵巢癌组织中表达的丰度差异无统计学意义(P>0.05),但卵巢癌组织中ERRγ的阳性表达率显著增高(P=0.045)。ERRs家族和ERs家族在卵巢癌中有很高的共同表达率,ERα/ERRα的比率在卵巢癌组织中明显下降(P=0.0412)。结论:ERRs与ERs在卵巢癌中有共同表达,两个家族间的相互作用可能是卵巢癌复杂的肿瘤内分泌生物学行为的分子基础。

The mRNA expression of human estrogen receptor-related receptor subfamily and estrogen receptor subfamily in ovarian cancer by using Real-time PCR

Objective:To determinate the co-expression pattern of human estrogen receptor-related receptors-α,-β and-γ,a subfamily of orphan receptors,and estrogen receptor subfamily in the ovarian cancer.Methods:Light Cycler PCR were used to analyze the expression of hERRα,hERRβ,hERRγ as well as ERα and ERβ mRNA in 40 primary ovarian cancer samples and 15 normal ovarian epithelial cells.Serum CA-125 was also assayed in all samples by ELISA.Results:Compared with normal ovarian tissues,ERRα was detected with a higher mRNA expression level and positive-ratio in ovarian cancer(P=0.04 and P=0.02).Nevertheless,the ERRβ was poorly expressed in ovarian cancer as well as normal tissues(P>0.05).Positive-ratio of ERRγ was also increased in ovarian cancer tissues than that in normal tissues(P=0.045),however,the mRNA expression level was similarly between ovarian cancer tissues and normal ovarian tissues(P>0.05).Moreover,the ratio of ERα/ERRα was observed decreasing in the ovarian cancer(P>0.05).Conclusion:ERRs and ERs play an important role in the development of ovarian cancer.ERRs may be the next endocrinal therapy target in ovarian cancer.