| 蜂毒素对大鼠血小板内钙浓度的影响 |
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| 引用本文： | 游育红,邬大元,黄自强. 蜂毒素对大鼠血小板内钙浓度的影响[J]. 福建医科大学学报, 1995, 0(4) |
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| 作者姓名： | 游育红 邬大元 黄自强 |
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| 作者单位： | 福建医学院药理学教研室，福建医学院生化中心实验室 |
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| 基金项目： | 福建省卫生厅青年科研基金 |
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| 摘 要： | 应用Ｆｕｒａ－２作为荧光试剂，测定大鼠血小板细胞内Ｃａ~（２＋）浓度变化。蜂毒素１．５ｍｇ／Ｌ使血小板细胞内Ｃａ~（２＋）浓度增加；悬液中加入ＣａＣｌ＿２１ｍｍｏｌ／Ｌ，血小板细胞内Ｃａ~（２＋）继续增加。维拉帕米３．１２５ｍｇ／Ｌ作用５ｍｉｎ后，蜂毒素仍可使血小板内Ｃａ~（２＋）增加．但加入ＣａＣｌ２血小板Ｃａ~（２＋）的浓度不再增加。提示蜂毒素促进大鼠血小板细胞内Ｃａ~（２＋）的释放和细胞外Ｃａ~（２＋）进入细胞内。
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| 关 键 词： | 血小板，Fura－a，蜂毒素，维拉帕米，Ca~（2＋） |
Effects of Melitten on the Intracelluar Inonic Calcium Concentration of Platelets of Rats |
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| You Yuhong,Wu Dayuan,Huang Ziqiang. Effects of Melitten on the Intracelluar Inonic Calcium Concentration of Platelets of Rats[J]. Journal of Fujian Medical University, 1995, 0(4) |
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| Authors: | You Yuhong Wu Dayuan Huang Ziqiang |
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| Abstract: | Fura-2AM was adopted as a fluorescence indicater to study the effects of melitten on the intracelluar free calcium concentration of platelets of rats.Melitten 1.5mg/L increased the intracelluar calcium concentration of platelets of rats.At this moment,CaCl_2 1 mmol/L was addedand the intracelluar calcium concentration increased further.The effects of the first step were not inhibited but those of the second step were inhibited by pretreatment with verapamil 3.125 mg/L in 5 min.It is suggested that melitten increases the release of intracelcular calcium and influx of calcium into platelets of rats. |
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| Keywords: | platelets Fura-2AM melitten verapamil calcium |
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