DiaMed单克隆凝胶血型卡在脐血血型血清学中的应用研究

目的探讨DiaMed单克隆凝胶血型卡在鉴定新生儿脐带血ABO、Rh（D）血型及进行直接抗人球球蛋白试验的可行性。方法取新生儿脐血样，采用试管法和DiaMed单克隆凝胶血型卡进行ABO和Rh（D）及进行直接抗人球球蛋白试验。结果在鉴定新生儿ABO血型时，试管法和DiaMed单克隆凝胶血型卡定型符合100％，Rh（D）检测结果两法完全一致。在直接抗人球球蛋白试验中，试管法的检出率为4．04％，DiaMed单克隆凝胶血型卡为5．05％。结论DiaMed单克隆凝胶血型卡在婴儿直接抗人球球蛋白试验时优于传统试管法，鉴定ABO和nh（D）血型结果可靠，易于标准化，在常规新生儿血型检查和直接抗人球球蛋白试验中可推广应用。

The study on the application of DiaMed odd clone gelatin cards in navel blood type serology

Objective To study the feasibility of the application of DiaMed add clone gelatin card in identifying ABO and Rh（D） of newborn＇ s navel blood and direct coombs＇ test. Methods Navel blood were samplied. In identifying ABO and Rh （D） were used DiaMed odd clone gelatin cards and test tube method and direct coombs＇ test. Results The results of identifying blood type and identifying Rh （D） were all identical between DiaMed odd clone gelatin cards and test tube method and in direct coombs＇ test, the checked ratio of test tube method was 4. 04% and the DiaMed odd clone gelatin cards＇ was 5.05%. Conclusion DiaMed odd clone gelatin cards is better than test tube method in direct coombs＇ test,and the identifying results of ABO and Rh（D） are reliable. It could have a good use in identifying newborn＇ s blood type and in direct coombs＇ test.