COX-2和VEGF-C表达与NSCLC淋巴转移的关系

目的探讨环氧化酶-2(COX-2)和血管内皮生长因子-C(VEGF-C)表达与淋巴管形成和淋巴结转移之间的关系。方法用免疫组化链霉素抗生物素蛋白-过氧化物酶(SP)法检测65例非小细胞肺癌(NSCLC)及16例正常肺组织中COX-2、VEGF-C及其受体VEGFR-3的表达;以VEGFR-3作标记计数肿瘤LVD,并结合临床病理特征进行统计学分析。结果65例NSCLC中COX-2和VEGF-C表达阳性率分别为76.9%(50/65)、72.3%(47/65)。COX-2表达与淋巴结转移(r=0.489,P<0.01)、临床分期(r=0.354,P<0.05)、VEGF0C(r=0.640,P<0.01)和LVD(r=0.518,P<0.01)呈正相关,而与病理分化程度呈负相关(r=-0.427,P<0.01)。VEGF-C表达与淋巴结转移(r=0.453,P<0.01)、临床分期(r=0.442,P<0.01)和LVD(r=0.624,P<0.01)呈正相关,与病理分化程度也呈负相关(r=-0.525,P<0.01)。结论NSCLC中COX-2与VEGF-C均高表达,COX-2可能通过VEGF-C促进肿瘤淋巴管生成和淋巴结转移。研究COX-2和VEGF-C在肿瘤中的协同作用有助于揭示肿瘤侵袭和转移的机制。

Expression of COX-2 and VEGF-C and their correlation with lymphatic metastasis in NSCLC

Objective To investigate the expression of COX-2 and VEGF-C and their correlation with lymphangiogenesis and lymph node metastasis in lung cancer.Methods The expression of COX-2,VEGF-C and their receptor,VEGFR-3 was detected in 65 patients with NSCLC and 16 normal pulmonary tissue specimens by immunohistochemical staining SP method.The lymphatic vessel density(LVD)in tumor was counted by VEGFR-3and analyzed with clinicopathologic parameters. Results The positive rate of COX-2 and VEGF-C was positively correlated with lymphatic node metastasis (r=0.489,P<0.01),clinical stage(r=0.354,P<0.05),VEGF-C(r=0.640,P<0.01)and LVD(r=0.518,P<0.01),but was negatively correlated with pathologic differentiation(r=0.427,P<0.01).The expression of VEGF-C was positively correlated with lymph node metastasis (r=0.354,P<0.01),clinical stage(r=0.442,P<0.05),LVD(r=0.624,P<0.01),and also negatively correllated with pathologic differentiation (r=0.525,P<0.01).Conclusion High expression of COX-2 and VEGF-C exist in NSCLC. COX-2 may stimulate lymphangiogenesis and lymphatic metastasis via VEGF-C, Study of their ynteraction will enormousely promote to discover mechanisms of tumorous invasion and metastasis.