抑制mdr1基因对人宫颈癌细胞放射敏感性影响的实验研究 |
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引用本文: | 任庆兰,吴永忠,陈晓品,李少林.抑制mdr1基因对人宫颈癌细胞放射敏感性影响的实验研究[J].现代妇产科进展,2008,17(5):346-349. |
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作者姓名: | 任庆兰 吴永忠 陈晓品 李少林 |
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作者单位: | 1. 重庆医科大学附属第一医院肿瘤科,重庆,400016 2. 重庆市肿瘤医院放疗科 3. 重庆医科大学基础医学院放射医学教研室 |
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基金项目: | 重庆医科大学科研课题启动基金 |
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摘 要: | 目的:探讨用反义技术抑制癌基因mdr1对宫颈癌细胞(HeLa)放射敏感性的影响。方法:设空白组、mdr1正义寡核苷酸组和脂质体组(仅加入等量的脂质体)为对照组以及mdr1反义寡核苷酸组,用RT-PCR检测宫颈癌细胞处理前后mdr1表达水平的变化。用集落形成试验检测各实验组照射后人宫颈癌HeLa细胞株集落形成率,AO/EB荧光染色法检测细胞凋亡率的变化,流式细胞仪测定Bcl-2蛋白的表达。结果:mdr1反义寡核苷酸能有效地抑制mdr1癌基因的表达,经脂质体介导的mdr1反义寡核苷酸作用的宫颈癌细胞经60Coγ射线照射后其集落形成率较正义寡核苷酸组及单纯照射组明显下降,而凋亡率明显增加,Bcl-2蛋白表达显著下调,与对照各组比较有显著差异(P<0.05)。结论:mdr1反义寡核苷酸通过抑制mdr1基因降低人宫颈癌HeLa细胞放射抗性。其辐射增敏作用可能与mdr1反义寡核苷酸增加照射后肿瘤细胞的凋亡率有关。
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关 键 词: | 宫颈肿瘤 HeLa细胞 mdr1基因 反义寡核苷酸 放射敏感性 |
Effect of inhibiting mdr1 on radiosensitivity in human cervical carcinoma cell line HeLa |
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Affiliation: | Ren Qinglan, Wu Yongzhong,Chen Xiaopin ,et al. (1. Department of Oncology,the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016;2. Department of Radiology, Chongqing Cancer Hospital) |
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Abstract: | Objective:To explore the effect of inhibiting mdrl by antisense technology on radiosensitivity in human cervical carcinoma cell line HeLa. Methods: There were four groups in the study : control group, lipofectin group, sense oligodexynucleotides (SODN) group, antisense oligodexynucleotides (ASODN) group. The expression of mdrl was detected by means of RT-PCR. Cellular response to irradiation was evaluated by the colony forming test. Apoptosis was observed by fluorescent staining. Bel-2 protein expression were determined by flow cytometry. Results:In AS-ODN group, the proliferation rate of irradiated HeLa decreased significantly and apoptosis increased and the expression of Bcl-2 was downregulated (P 〈 0.05 ). Conclusion:mdrl antisense oligodexynucleotides sensitize the HeLa to ionizing irradiation through decreasing the expression of mdrl ,which may be related to increasing the apoptosis rate of HeLa cells. |
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Keywords: | Cervix neoplasms HeLa cells mdrl gene Antisense oligomucleotides Radiosensitivity |
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