荧光定量聚合酶链反应检测CCND1基因扩增在乳腺癌诊断中的应用

目的应用荧光定量聚合酶链反应（FQ-PCR）法检测细胞周期蛋白D1（CCND1）基因表达水平，探讨其在乳腺癌诊断和治疗监测中的应用价值。方法应用FQ-PCR法，并以β2-微球蛋白为内对照测定15例健康女性体检者、30例良性乳腺疾病患者和81例乳腺癌患者外周血中CCND1的表达量。结果CCND1表达水平在健康对照组和良性乳腺疾病组间差异无统计学意义（P〉0．05），乳腺癌组均高于前2组（P〈0．05），β2-微球蛋白在3组间差异无统计学意义（P〉0．05）。81例乳腺癌患者阳性率为45．7％，良性乳腺疾病组为0。结论FQ-PCR技术是高度灵敏、高度特异的快速定量检测CCND1方法，可有效监测乳腺癌的诊断：疗效、转移，评估其预后。

Clinical application of CCND1 gene expression detection with fluorescent quantitative polymerase chain reaction in the diagnosis of breast cancer

Objective To use fluorescent quantitative polymerase chain reaction (FQ-PCR) todetect the level of cyclin D1 gene (CCND1), and to investigate its clinical application in the diagnosisand monitoring of breast cancer. Methods FQ-PCR was applied to detecting the expression level ofCCND1 gene in peripheral blood from 15 healthy females, 30 patients with benign breast disease and81 patients with breast cancer with β2-microglobin as the internal control. Results There were no sig-nificant difference in the level of CCND1 expression between healthy control group and benign breastdisease group (P>0. 05). The level of CCND1 expression was significantly higher in breast cancergroup than that in healthy control group and benign breast disease group (P<0.05). The difference inβ2-microglobin was statistically insignificant among three groups (P> 0. 05). The positive rate was45.7% in breast cancer group, and 0 in benign breast disease group. Conclusion FQ-PCR is a rapid,sensitive and specific method for quantitative determination of CCND1 gene, which may he used as anavailable tool for the diagnosis, monitoring of therapeutic effect and metastasis as well as prognosis e-valuation of breast cancer.