嗅鞘细胞上清液对抗过氧化氢诱导的星形胶质细胞损伤的线粒体机制

目的： 研究嗅鞘细胞上清液（olfactory ensheathing cell conditioned medium, OECCM）对星形胶质细胞的保护作用及其线粒体机制。方法： 先用500 μmol/L H₂O₂ 损伤星形胶质细胞20 min；再以50%的OECCM继续培养细胞24 h。MTT法检测星形胶质细胞的活性，Hoechst 33342 染色法和蛋白质印迹法检测细胞凋亡及凋亡相关蛋白caspase-3的表达；用JC-1染色法检测线粒体的膜电位。结果： OECCM能减少H₂O₂诱导的细胞凋亡，减少caspase-3的表达，并且使线粒体膜电位增高。结论： OECCM可以对抗500 μmol/L H₂O₂诱导的星形胶质细胞凋亡，其机制可能与稳定线粒体膜电位有关。

Mitochondria mechanism of olfactory ensheathing cells conditioned medium against H2O2 induced injury in astrocytes

Objective: To study the mitochondria mechanism of olfactory ensheathing cells conditioned medium(OECCM) against H2O2 induced injury in astrocytes.Methods: After treatment of 500 μmol/L H2O2 for 20 min,50% OECCM was added to treat the cells for 24 h;MTT assay was used to analyse astrocytes viability;apoptosis induced by 500 μmol/L H2O2 was decreased by hoechst 33342 staining and the expression of caspase-3 by Western blot;mitochondrial membrane potential was detected by JC-1 staining.Results: OECCM could protect astrocytes from the injury of 500 μmol/L H2O2;apoptotic cells were decreased and the expression of caspase-3 was decreased also;OECCM could increase mitochondrial membrane potential.Conclusion: OECCM could against H2O2 induced injury in astrocytes.Its mechanism might be related to stabilize mitochondria function.