慢性粒细胞白血病患者HAGE基因启动子的低甲基化

目的： 探讨中国人群慢性粒细胞白血病（chronic myeloid leukemia,CML）患者螺旋酶抗原(helicase antigen,HAGE)基因启动子的甲基化状况和临床相关性。方法： 应用甲基化特异性PCR（methylation specific PCR，MSP）技术对50例不同临床分期的CML患者骨髓标本HAGE基因启动子的甲基化状态进行检测。结果： 13例（26%）CML患者存在HAGE基因启动子的低甲基化改变，而24例对照均无HAGE基因低甲基化，两组比较差异有统计学意义（P=0.007)。HAGE基因启动子低甲基化改变与CML患者的年龄、性别、白细胞计数、血红蛋白含量、血小板计数、临床分期及染色体异常均无相关性（P>0.05）。慢性期、加速期和急变期患者中HAGE基因启动子低甲基化频率分别为25%（9/36）、25%（1/4）和30%（3/10）（P>0.05）。结论： HAGE基因启动子低甲基化是CML中的常见分子事件。

Hypomethylation of HAGE gene promoter in patients with chronic myeloid leukemia

Objective: To study the methylation status of helicase antigen(HAGE) gene promoter and its clinical correlations in Chinese patients with chronic myeloid leukemia(CML).Methods: The methylation status of HAGE promoter in the bone marrow samples from 50 CML patients in different stages was detected using methylation-specific PCR(MSP) technique.Results: Hypomethylation of HAGE gene promoter was found in 13(26%) CML cases,but not in all 24 controls(P=0.007).There was no correlations between the hypomethylation of HAGE gene promoter with the age,sex,white blood cell count,hemoglobin concentration,platelet count,clinical stages,and chromosomal abnormalities of CML patients(P>0.05).The frequencies of HAGE promoter hypomethylation in patients in chronic phase or accelerated phase,and in blast crisis were 25%(9/36),25%(1/4) and 30%(3/10),respectively(P>0.05).Conclusion: Hypomethylation of HAGE gene promoter was a frequent molecular event in CML.