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小鼠海马CA1区锥体神经元树突棘的发育
引用本文:刘畅,范文娟,程维杰,左曙光,邓锦波. 小鼠海马CA1区锥体神经元树突棘的发育[J]. 解剖学报, 2012, 43(1): 1-6. DOI: 10.3969/j.issn.0529-1356.2012.01.001
作者姓名:刘畅  范文娟  程维杰  左曙光  邓锦波
作者单位:(1.漯河医学高等专科学校分子医学实验中心,河南 漯河462002; 2.河南大学神经生物学研究所,河南 开封475004)
摘    要:目的 对小鼠海马CA1区锥体神经元正常发育中树突棘密度及各种形态变化进行分析测定,为深入研究突触发生及突触可塑性提供直接的形态学依据.方法 分别取出生后0、5、10、20及30d 5个年龄段的C57BL/6小鼠各10只,采用基因枪对小鼠海马CA1区锥体神经元树突棘进行亲脂性荧光染料DiI标记,通过激光共焦显微镜对其进行观察分析;同时利用透射电镜技术对树突棘的超微结构进行分析.结果 树突棘的形态、大小及其密度随小鼠发育而变化,成熟树突棘内部存在滑面内质网与棘器,可能参与了突触后膜结合蛋白及其转运体的合成.结论 树突棘的发育过程与突触连接的形成以及突触可塑性密切相关.

关 键 词:树突棘  突触  海马  免疫荧光  透射电镜  小鼠
收稿时间:2011-07-21
修稿时间:2011-08-30

Dendritic spine development of mouse hippocampal CA1 pyramidal neurons
LIU Chang,FAN Wen-juan,CHENG Wei-jie,ZUO Shu-guang,DENG Jin-bo. Dendritic spine development of mouse hippocampal CA1 pyramidal neurons[J]. Acta Anatomica Sinica, 2012, 43(1): 1-6. DOI: 10.3969/j.issn.0529-1356.2012.01.001
Authors:LIU Chang  FAN Wen-juan  CHENG Wei-jie  ZUO Shu-guang  DENG Jin-bo
Affiliation:(1.Experimental Center of Molecular Medicine, Luohe Medical Colledge, He’nan Luohe 462002, China; 2.Institute and Laboratory of Neurobiology, He’nan University, He’nan Kaifeng 475004, China)
Abstract:[Abstract] Objective To investigate the developmental characteristics of dendritic spines in mouse hippocampal CA1 pyramidal neurons by analysing the spine density and morphological changes. Methods Fifty mice were collected at postnatal days (P) 0, 5, 10, 20 and 30, 10 mice for each age. DiI diolistic labeling with gene gun was performed to observe dendritic spines development in mouse hippocampal CA1 pyramidal neurons. High quality labeled neurons were examined and photographed under a confocal microscope, whereas the ultrastructure of spines was observed under a transmission electron microscope. Results Dendritic spines changed their morphology and density with mouse development in response to neuronal activity. The smooth endoplasmic reticulum and spine apparatus in dendritic spines of hippocampal CA1 were observed with electron microscopy analysis, which might be involved in the regulation of plasticity at individual synapses.Conclusion The development of dendritic spines may be closely related to synaptogenesis and the
Keywords:Dendritic spine  Synapse  Hippocampus  Immunofluorescence  Transmission electron microscopy  Mouse
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