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人组织型纤溶酶原激活剂(t-PA)与绿色荧光蛋白(GFP)在转基因烟草中的融合表达
引用本文:王庆华,王永中,陈宇坤,张新元,叶波平,奚涛.人组织型纤溶酶原激活剂(t-PA)与绿色荧光蛋白(GFP)在转基因烟草中的融合表达[J].药物生物技术,2004,11(2):76-80.
作者姓名:王庆华  王永中  陈宇坤  张新元  叶波平  奚涛
作者单位:中国药科大学生物技术中心,江苏,南京,210009
摘    要:构建人组织型纤溶酶原激活剂(t-PA)与绿色荧光蛋白(GFP)融合表达的植物表达载体pCA1302NSF-tPA。将重组载体转入根瘤农杆菌GV3101中,通过叶圆盘法转化烟草。转化植株经PCR鉴定整合有t-PA基因,通过荧光显微镜可检测到绿色荧光蛋白(GFP)的表达。

关 键 词:人组织型纤溶酶原激活剂  绿色荧光蛋白  融合表达  转基因烟草
文章编号:1005-8915(2004)02-0076-05

Expression of Human Tissue-Type Plasminogen Activator and Green Fluorescent Fusion Protein in Transgenic Tobacco
WANG Qing-hua,WANG Yong-zhong,CHEN Yu-kun,ZHANG Xin-yuan,YE Bo-ping,XI Tao.Expression of Human Tissue-Type Plasminogen Activator and Green Fluorescent Fusion Protein in Transgenic Tobacco[J].Pharmaceutical Biotechnology,2004,11(2):76-80.
Authors:WANG Qing-hua  WANG Yong-zhong  CHEN Yu-kun  ZHANG Xin-yuan  YE Bo-ping  XI Tao
Abstract:Fusion gene of human tissue-type plasminogen activator(t-PA) and green fluorescent protein(GFP) was inserted into pCAMBI1302 for constructing the plant expressing vector pCA1302NSF-tPA.Then the recombinant vector was transformed into A.tumefaciens GV3101 and the fusion gene was transformed into Nicotina tobaccum L.W38 line with the help of GV3101.Transgenic tobacco plants were detected by PCR method and the result shows that t-PA cDNA was integrated into the genome of tobacco.The recombination t-PA-GFP could be detected through fluorescent telescope in leaf and its protoplasts. So a effective and rapid way to screen positive transgenic tobacco plants was established.
Keywords:t-PA  GFP  Fusion expression  Transgenic tobacco
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