秋水仙碱对心肌组织中TGF-β_1的影响

目的 探讨秋水仙碱对压力负荷性大鼠心肌中转化生长因子β_1(TGF-β_1)的影响.方法 利用腹主动脉缩窄的方法 建立压力超负荷性心肌大鼠模型,放射免疫分析法测定局部心肌组织中血管紧张素Ⅱ(Ang Ⅱ)水平,RT-PCR法半定量TGF-β_1mRNA的表达变化,免疫组织化学法测定心肌TGF-β_1的表达,同时给予秋水仙碱8周,观察上述指标的变化.结果 与对照组相比,模型组AngⅡ、TGF-β_1 mRNA和TGF-β_1 表达明显增加(P<0.01),秋水仙碱能明显减少上述指标的表达.结论 秋水仙碱可以通过抑制压力负荷性心室重构大鼠RAAS和交感神经系统活性,减少神经内分泌因子AngⅡ生成,减少纤维化因子TGF-β_1的表达.

Effect of colchicine on myocardium transforming growth factor-beta 1

Objective To investigate the effect of colchicine on myocardium β_1.Methods The model was induced by abdominal aorta constriction(AC)in rats.After 8 weeks treatment,cardiac muscle AngⅡwere measured by radioimmunoassay,and ventricular transforming growth factor-beta 1(TGF-β_1)was examined bv RT-PCR,and the content of TGF-β_1 in myocardium were determined using immunohistochemical analysis after AC.Results The levels of Ang Ⅱ and TGF-β_1 mRNA and the content of TGF-β_1 were significantly increased after AC eight weeks and significantly decreased after treatment with colchicine.Conclusion Colchicine can significantly attenuate the level of myocardium TGF-β_1;the mechanism is related with its ability to inhibit the activation of rennin-angiontensin-aldosterone system (RAAS)and systema nervosum sympatheticum(SNS),decrease the content of Ang Ⅱ and TGF-β_1.