纤维粘连蛋白在介导白念珠菌与宿主细胞粘附中的作用

为了弄清纤维粘连蛋白（Fn）在介导白念珠菌（简称白念）与宿主细胞粘附中的作用，把人颊粘膜上皮细胞与白念孢子共同振荡孵育2小时，显微镜下测定上皮细胞表面粘附的白念数目，粘附20个以上的上皮细胞记为+，共检测100个，计算百分比为44．75±6．82，再将纤维粘连蛋白（Fn）与上皮细胞和白念共同孵育，具体方法同上，百分比为26.25±5.06，P＜0.01。验证了Fn可能介导上皮细胞与白念的粘附。从受体水平探讨了白念与宿主细胞的粘附机制，为防治白念感染提供了新的途径。

The effect of fibronectin on Candida albicans adherence to host cells

To find out the effect of fibronectin on Candida albicans adherence to hostcells, human buccal epithelial cells and Candida albicans yeast cells were incubated at37C for 2h on a rotator, and yeasts adhering to the surfaces of epithelial cells were as-sayed microscopically. Epithelial cells with> 20 yeasts were considered adhesive, and their number per 100 cells scanned yielded the percentage of adhesive epithelial cells. Itwas 44. 75 + 6. 82. Fibronectin was added to the adhesion mixture, and the reaction wasperformed as described ahove. It was 26. 25 + 5. 06, P < 0. 01. The data proved thatfibronectin could mediate adherence of Candida albicans to epithelia1 cells. These ex-periments studied adherence mechanism of Candida albicans in molecular level, and of-fered new ways to prevent and treat the infection.