人宫颈癌细胞的体外培养及鉴定

背景:由于肿瘤存在个体差异,对癌株的研究不能准确反映个体之间的差异,原代细胞培养则与体内状态更相近。目的:体外培养建立宫颈癌细胞原代培养方法。方法:采用胰蛋白酶及Ⅰ型胶原酶联合消化法从23例新鲜宫颈癌组织获取宫颈癌细胞,测定其细胞生长曲线,并用免疫荧光染色检测细胞表面标记物CD44、CK17进行鉴定。结果与结论:23例中有21例成功从宫颈癌组织中获取宫颈癌细胞并能连续传代,稳定增殖,免疫荧光染色显示宫颈癌细胞表面标记物CD44、CK17表达呈阳性。说明胰蛋白酶及Ⅰ型胶原酶联合消化法能成功分离原代宫颈癌细胞。

Culture in vitro and identification of human cervical carcinoma cells

BACKGROUND: There are individual differences in the patients suffered tumor. Therefore the research on the cancer line can not show accurate differences between individuals, while primary cell culture can show more similar characteristics to in vivo state. OBJECTIVE: To establish a method in vitro for primary culture of cervical carcinoma cells. METHODS: Cervical cancer cells were obtained from 23 fresh cervical cancer tissues using the trypsin and collagenase type Ⅰdigestion, and their growth curve was detected. Cell surface markers of CK17and CD44 were identified with immunofluorescence staining. RESULTS AND CONCLUSION: Cervical cancer cells were obtained from 21 of 23 cases, and the cells had the continuous passage and steady proliferation. Immunofluorescence staining showed that the cells positively expressed CK17 and CD44. It is indicated that primary cervical carcinoma cells can be obtained by trypsin and collagenase type Ⅰdigestion.