2型糖尿病合并急性脑梗死外周血CD34~+水平变化及其意义

背景:CD34+细胞是一种新的血管内皮标志物,通过检测CD34+细胞水平可以反映血管内皮功能的变化。目的:分析外周血CD34+细胞数量变化与脑梗死发病的关系。方法:纳入观察对象110例,分为4组,2型糖尿病组、急性脑梗死组、2型糖尿病合并急性脑梗死组各30例,健康对照组20例。应用流式细胞仪ProCOUNT方法测定外周血CD34+/外周血单核细胞值,分析外周血CD34+/单核细胞值与脑血管病危险因素的相关性。结果与结论:4组外周血CD34+/单核细胞值经过方差分析,差异具有显著性意义(P<0.05),健康对照组高于2型糖尿病组、急性脑梗死组、2型糖尿病合并脑梗死组(P<0.01),2型糖尿病组高于急性脑梗死组、2型糖尿病合并脑梗死组(P<0.01)。2型糖尿病合并急性脑梗死患者外周血CD34+/单核细胞值与空腹血糖和收缩压呈负相关,与高密度脂蛋白胆固醇/低密度脂蛋白胆固醇的比值呈正相关,与舒张压、吸烟、胆固醇、三酰甘油无相关性。提示CD34+细胞可能使脑血管病危险因素受到影响从而参与了脑梗死的发生,监测CD34+细胞水平变化也许能用来预测2型糖尿病患者急性脑梗死的发生。

Changes of peripheral blood CD34+ level in type 2 diabetes combined with acute cerebral infraction and its clinical significance

BACKGROUND: CD34+ cell is a new marker of vascular endothelium, the detection of CD34+ cells level may reflect the changes of endothelial function. OBJECTIVE: To analyze the relationship between the number of CD34+ cells and the cerebral infarction. METHODS: A total of 110 subjects were included to review, the subjects were divided into four groups: type 2 diabetes group, acute cerebral infraction group, combination group (n=30) and control group (n=20). Flow cytometry Pro COUNT method was used to measure the number of peripheral blood CD34+ mononuclear cells and analyze the relationship with the risk factors for cerebrovascular disease. RESULTS AND CONCLUSION: There was significant difference in the number of peripheral blood CD34+ mononuclear cells of four groups (P < 0.05) analyzed by variance. The number of peripheral blood CD34+ mononuclear cells in control group was higher than that in other three groups (P < 0.01), the type 2 diabetes group was higher than acute cerebral infraction group and combination group (P < 0.01). The number of peripheral blood CD34+ mononuclear cells in patients with type 2 diabetes combined with acute cerebral infraction was negative correlated with fasting plasma glucose and systolic blood pressure, and positively correlated with the ratio of high-density lipoprotein cholesterol/low-density lipoprotein cholesterol. It had no relationship with diastolic blood pressure, smoking, cholesterol and triglyceride. CD34+ cells can influence the cerebral infarction development by its risk factors, so the monitoring of CD34+ can be used to prevent the occurrence of acute cerebral infarction in type 2 diabetes patients.