表皮生长因子受体Ⅲ型突变体在原发性胆囊癌中的表达及其意义

目的 检测表皮生长因子受体Ⅲ型突变体(EGFRvⅢ)在原发性胆囊癌中的表达,探讨其与胆囊癌发生的关系.方法 采用免疫组化SABC法检测68例原发性胆囊癌病人中EGFRvⅢ的表达水平,并运用免疫组化图像分析法进行半定量.结果 原发胆囊癌组织EGFRvⅢ表达的总阳性率为53%,其中腺癌的阳性率为51%.依据病理分级.EGFRvⅢ表达水平由高到低分别为Ⅲ级>Ⅱ级>Ⅰ级,其差异具有统计学意义(q=2.49,P=0.02,q=3.63,P<0.01,q=5.69,P<0.01).不同性别者EGFRvⅢ表达水平无显著差异(t=1.29,P=0.20);EGFRvⅢ表达与年龄无显著相关性(r=0.03,P=0.88).结论 EGFRvⅢ在原发性胆囊癌组织存在高表达.对胆囊癌的发生、发展起一定作用.

Expression and significance of epidermal growth factor receptor variant Ⅲ in primary gallbladder carcinoma

Objective To detect expression of epidermal growth factor receptor variant Ⅲ(EGFRvⅢ)and investigate its significance in development of primary gallbladder carcinoma. Methods Sixty-eight patients with primary gallbladder carcinoma entered this study. Immunohistoehemistry (SABC assay) and image analysis were used to semiquantitatively detect the expression of EGFRvⅢprotein. Results Positive expression rate of EGFRvⅢ was 53% in primary gallbladder carcinoma and 51% in adenocarcinoma. As cancer pathologic grading advanced, the expression of EGFRvⅢ increased with statistical deviation (q=2.49, P=0.02, q=3.63, P<0.01, q=5.69, P<0.01). EGFRv Ⅲexpression level showed no statistical deviation between male and female subjects(t=1.29, P=0.20).No significant relationship was observed between EGFRvⅢ expression and age(r=0.03, P=0.88).Conclusion EGFRvⅢ is overexpressed in primary gallbladder carcinoma and may play certain role in development of primary gallbladder carcinoma.