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| 牙釉质和牙本质核DNA在法医个人识别中的应用研究 | |
| 引用本文： | 梁芸丹,向超杰,兰柳萍,余建华,张东,许冰莹.牙釉质和牙本质核DNA在法医个人识别中的应用研究[J].昆明医学院学报,2009,30(3):5-8. |
| 作者姓名： | 梁芸丹 向超杰 兰柳萍 余建华 张东 许冰莹 |
| 作者单位： | 1. 昆明医学院法医学院,云南,昆明,650031 2. 昆明市公安局,云南,昆明,650031 3. 昆明医学院口腔学院,云南,昆明,650031 4. 临沧市公安局,云南,临沧675800 |
| 基金项目： | 云南省应用基础研究项目 |
| 摘 要： | 目的评价牙釉质和牙本质核DNA在法医个人识别中的应用价值．方法从受外界理化因素和生物因素影响小的牙釉质、牙本质中提取核DNA，采集了20颗无关个体磨牙的牙釉质、牙本质用有机法提取核DNA．结果牙釉质、牙本质中能提取到理想的核DNA．结论牙釉质、牙本质中的细胞核DNA能用于法医个人识别，为实际案件的侦破提供重要证据． |
| 关 键 词： | 牙釉质 牙本质 核DNA提取 个人识别 法医学应用 |
Application of Nuclear DNA of Enamel and Dentin in PersonalIdentification |
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| LIANG Yun-dan,XIANG Chao-jie,LAN Liu-ping,YU Jian-hua,ZHANG Dong,XU Bing-ying.Application of Nuclear DNA of Enamel and Dentin in PersonalIdentification[J].Journal of Kunming Medical College,2009,30(3):5-8. | |
| Authors: | LIANG Yun-dan XIANG Chao-jie LAN Liu-ping YU Jian-hua ZHANG Dong XU Bing-ying |
| Institution: | LIANG Yun - dan , XIANG Chao -jie , LAN Liu - ping , YU Jian - hua , ZHANG Dong , XU Bing - ying (1) Dept. of Forensic Medicine, Kunming Medical University, Kunming Yunnan 650031; 2 ) Institute of Criminal Science and Technology of Kunming Police Bureau, Kunming Yunnau 650031 ; 3 ) Dept. of Stomatology, Kunming Medical University, Kunming Yunnan 650031, China) |
| Abstract: | Objective To evaluate the application of nuclear DNA of enamel and dentin in personal identification. Methods We extracted nuclear DNA from enamel and dentin, because enamel and dentin had little influence in physical and chemical factors. We collected 20 molars from 20 irrelevant persons. Results The traditional organic extraction method extracted cell nuclear DNA from Enamel and dentin, and obtained favourable cell nucleus DNA. Conclusion Nuclear DNA obtained from enamel and dentin provide important evidence for case. |
| Keywords: | Enamel Dentin Extraction of cell nuclear DNA Personal identification Forensicmedicine appliciation |
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