核酸检测与酶联免疫吸附试验在血液标本内乙肝病毒检测中的价值

目的 研究核酸检测(NAT)、酶联免疫吸附试验(ELISA)在血液标准内乙型肝炎(乙肝)病毒(HBV)检测中的价值.方法 20140例无偿献血者的血液标本为研究对象,先进行ELISA检测,对ELISA无反应性的标本再进行NAT,跟踪随访并分析检测结果.结果 20140份血液标本中,ELISA检测双试剂阳性6份,单试剂阳...

Value of nucleic acid testing and enzyme-linked immunosorbent assay in detection of hepatitis B virus in blood specimen

Objective To study the value of nucleic acid testing(NAT)and enzyme-linked immunosorbent assay(ELISA)in detection of hepatitis B virus(HBV)in blood specimen.Methods Blood specimens from 20140 unpaid blood donors were used for the study,and ELISA was performed first,followed by NAT for ELISA non-reactive specimens,and the test results were followed up and analyzed.Results Among the 20140 blood specimens tested,6 specimens were positive for double reagents,8 specimens were positive for single reagents,and 20126 specimens were negative for double reagents by ELISA.Among the double-reagentnegative specimens,20111 specimens were negative and 15 specimens were positive by NAT testing.Among the 15 confirmed positive samples,the blood donors meeting the diagnostic criteria were followed up,and 3 cases with positive ELISA negative conversion were confirmed by NAT testing in the ELISA window period.2 cases of occult HBV infection were found in NAT testing.Conclusion NAT testing has the advantages of high sensitivity and specificity in clinical detection,and it is complementary to ELISA to a certain extent.It is suitable for blood examination,and can significantly reduce the occurrence of misdiagnosis and reduce the window period,which is of great significance to the safety of blood transfusion.