2017-2020年湖北省甲型H3N2亚型流感病毒流行特征和进化分析

目的 了解2017-2020年湖北省甲型H3N2亚型流行性感冒(流感)流行分布及基因进化情况.方法 根据湖北省流感病原学监测数据,分析2017-2020年甲型H3N2亚型流感病毒流行分布情况,按年度分布和地域分布的原则选取2017-2020年各市级流感监测网络实验室送检的该亚型流感病毒共38株进行测序,获得病毒HA和N...

Epidemiological and evolutionary analysis on influenza A (H3N2) virus in Hubei,2017-2020

  Objective  To investigate the epidemiological distribution and gene evolution of influenza A (H3N2) virus in Hubei province from 2017 to 2020.  Methods  Based on the influenza etiological surveillance data in Hubei, the epidemiological distribution of influenza A (H3N2) virus in Hubei during 2017–2020 was analyzed. A total of 38 strains of influenza A (H3N2) virus sent by the influenza surveillance network laboratories in Hubei during 2017–2020 were selected for gene sequencing according to the annual distribution and area distribution. The amino acid sequences of HA and NA were obtained and their antigens were analyzed, the changes of amino acid sites in clusters were analyzed, and gene evolution and 3D modeling analyses were conducted.  Results  There were three detection peaks of influenza A (H3N2) virus in Hubei from 2017 to 2020. The first peak was predominated by 3C.2a1 virus cluster, the second peak was predominated by 3C.2a1b+T131K virus cluster and the third peak was predominated by 3C.2a1b+T135K virus cluster. 3C.2a1b +T131K virus cluster and 3C.2a1b+T135K virus cluster had 8 different amino acid mutation sites on 3 antigenic determinants of HA protein. There were significant differences among sites 50, 131, 135, 140 of 3C.2a1b+T131K virus cluster and 3C.2a1b+T135K virus cluster in the three-dimensional simulation structure diagram.  Conclusion  The cluster of influenza A (H3N2) virus in Hubei evolved continuously from 2017 to 2020. Enhancement of evolutionary surveillance and antigen mutation analysis of the recent cluster of 3C.2a1b+T135K virus would improve the epidemiological and gene evolution surveillance of influenza viruses in Hubei.