阿魏酸钠对结肠炎大鼠结肠组织一氧化氮合酶和环氧合酶的影响

目的　研究阿魏酸钠 (SF)对大鼠结肠炎的抗炎保护作用及其机制。方法　建立大鼠乙酸性结肠炎模型。SF与5 氨基水杨酸灌肠给药 1wk后评价大鼠结肠黏膜损伤指数(CMDI) ,采用试剂盒及免疫组化方法检测结肠组织NO、MPO、PGE2 含量及结构型一氧化氮合酶 (cNOS) ,诱生型一氧化氮合酶 (iNOS) ,环氧合酶 1(COX 1) ,环氧合酶 2 (COX 2 )和核因子 κBp6 5 (NF κBp6 5 )的表达水平。 结果　SF(2 0 0、4 0 0、80 0mg·kg-1)灌肠用药均降低模型组大鼠升高的CMDI及结肠组织NO、MPO、PGE2 含量 ,下调iNOS、COX 2、NF κBp6 5的过度表达 ,亦抑制cNOS的正常表达水平 ,对COX 1表达影响不明显。SF用药呈现一定量效关系。结论　SF为一氧化氮合酶及部分选择性COX 2抑制剂 ,对大鼠结肠炎具有一定抗炎保护作用。

Effects of sodium ferulate on nitric oxide synthase and cyclooxygenase in colon of colitis rats

AIM To investigate the anti-inflammation protective effects of sodium ferulate (SF) on colitis rats and its mechanism. METHODS The colitis model of rats was produced by intracolon enema with acetic acid. SF and 5-ASA were used intracolonically for a week. The colon mucosadamage index (CMDI) was evaluated. Nitric oxide (NO), myelopexoxidase (MPO), prostaglandin (PGE_2), and the levels of expression of constitutive nitric oxide synthase(cNOS), induce nitric oxide synthase (iNOS), cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2) and nuclear factor-kappaBp65(NF-κBp65) in the rats colon were detected by corresponding kits and immunohistochemical technology. RESULTS SF (200,400,800 mg·kg -1 ) can decrease the extents of CMDI and the levels of NO, MPO, PGE_2, the expression of cNOS, iNOS, COX-2, and NF-κBp65 in model group in a dose-dependent manner while the expression of COX-1 changes littlely. CONCLUSION SF is a NOS and partial selective COX-2 inhibitor and show therapeutic effect on colitis in rats.