人源和猪源猪链球菌的同源性研究

目的 进一步鉴定菌种；评价人源株与猪源株猪链球菌的同源性。方法 对分离自猪无菌部位、患者血液和脑脊液的７株猪链球菌Ⅱ型和标准猪链球菌Ⅱ型，用菌体脂肪酸分析和随机引物基因扩增技术进行菌种的表现型和基因型分类。对其结果进行聚类分析和主成分分析。结果 随机引物基因扩增技术分析提示，被检的７株猪链球菌Ⅱ型与标准株一致，均为猪链球菌Ⅱ型；人源株与猪源株同源；分离自病人血液和脑脊液的菌株同源。这些结果在菌体脂

Homogeneity study on the Streptococcus suis isolated from human and swine

Objective To identify S.suis and to evaluate the homogeneity of isolates of S.suis from human and swine. Methods Culture, morphology, API biochemical tests and serum coagulate tests were used. All the 7 strains of streptococcus were confirmed to be S.suis serotype 2. Two strains and 1 strain were isolated from blood and cerebrospinal fluid (CSF) of patients, respectively, while the other 4 strains were obtained from the infected swine or their corpses. Seven strains of S.suis serotype 2 and reference strain SS2 were analyzed by randomly amplified polymorphic DNA analysis with six primers and Rep PCR with four primers and thalli fatty acid profile analysis. Cluster and principal compound analysis of results were performed with RAPD, Phylip and Treeview software. Results By analysis on RAPD patterns, a close relationship among 7 strains of S.suis serotype 2 and reference strain SS2 was discovered. Human born strains and swine born strains exhibit similar RAPD patterns. There was a same clonal relationship between strains originated from the blood and CFS of the patients. These results were confirmed by thalli fatty acid profile to have genotypic and phenotypic identity. Conclusion Homogeneity exists among the 7 strains and reference strain SS2.