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| 荧光PCR熔解曲线法检测结核分枝杆菌耐利福平突变研究 | |
| 引用本文： | 马艳艳,李辉,赵东阳,李庆阁.荧光PCR熔解曲线法检测结核分枝杆菌耐利福平突变研究[J].口岸卫生控制,2011,16(5):21-24. |
| 作者姓名： | 马艳艳 李辉 赵东阳 李庆阁 |
| 作者单位： | 1. 河南国际旅行卫生保健中心 河南,郑州,450046 2. 河南省疾病预防控制中心 河南,郑州,450016 3. 厦门大学 福建,厦门,361012 |
| 摘 要： | 目的 对荧光PCR熔解曲线法检测结核分枝杆菌耐利福平突变方法进行临床研究,评价其检测效能及在国境口岸应用价值.方法 应用荧光PCR熔解曲线法检测结核病人临床分离标本,与传统药物敏感性试验对比,衡量该方法的灵敏度、特异性.结果 对347例结核病人临床分离培养样本,药敏试验检出271例利福平敏感标本,76例利福平耐药标本；... |
| 关 键 词： | 结核分枝杆菌 熔解曲线法 利福平 检测 |
Detecting the mutation of ropB gene in mycobacterium tuberculosis isolates from clinical sample by melting analysis based real-time PCR |
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| Authors: | Ma Yanyan Li Hui Zhao Dongyang Li Qingge |
| Institution: | Ma Yanyan Henan international travel healthcare center(Henan,Zhengzhou,450046) Li Hui Zhao Dongyang Henan centers for disease control and prevention(Henan,450016) Li Qingge Xiamen university(Fujian,Xiamen,361012) |
| Abstract: | Objective To elevate the analysis abilities and valuation of the melting analysis based real-time PCR for detecting the mutation of ropB gene in mycobacterium tuberculosis in frontier health quarantine. Methods To compare the melting analysis assay and drug susceptibility testing (DST) by detecting the isolates from clinical sample. Results 347 mycobacterium tuberculosis isolates were detected by two methods, 271 rifampin sensitivity isolates and 76 rifampin resistant isolates were gained by DST, 269 rifamp... |
| Keywords: | Mycobacterium tuberculosis melting analysis assay rifampin detect |
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