白细胞介素13基因在人肾组织的表达及其意义

目的 探讨白细胞介素13（IL-13）基因在原发性非IgA系膜增生性肾炎（MsPGN）、原发性局灶节段性肾小球硬化症（FSGS）和狼疮肾炎（LN）患者肾组织的表达及意义。方法 采用原位杂交技术（ISH）检测34例上述3种肾脏病患者及4例对照组肾组织中IL-3 mRNA表达量的变化，分析IL-3基因表达量与肾功能变化的关系。结果 IL-13 mRNA在FSGS、MsPGN和LN患者肾组织内表达均增高，而对照者肾组织内无明显IL-3 mRNA表达。LN患者肾小管间质区IL-3 mRNA表达水平与狼疮活动相关，MsPGN及PSGS患者肾小管间质区IL-13 mRNA表达量与肾功能变化及24h尿蛋白量相关。结论 IL-13可能直接参与人类肾小球疾病的免疫反应过程。

Interleukin-13 gene expression in renal tissne of human kidney diseases

Objective To study the clinical significance of interleukin-13 (IL-13 ) gene expression in renal tissue of human kidney diseases. Methods 4 controls and 34 patients with primary focal segmental glomerular sclerosis (FSGS), non-IgA primary mesangioproliferative glomerulonephritis(MsPGN) and lupus nephritis(LN) were included in this study. IL-13mRNA expression in renal tissue was detected by in situ hybridization(ISH) technique. Results Up-regulating expressions of IL-13mRNA in the renal tissues of FSGS, MsPGN and LN were found, whereas little expression of IL-13mRNA in the renal tissue of the controls was observed. The elevated expression of IL-13mRNA in tubulointerstitium was closely related to lupus activity in LN. The levels of IL-13mRNA in tubulointerstitium correlated to the renal function and urinary albumin excretion in FSGS and MsPGN. Conclusion IL-13 may be involved in the immune reaction of renal tissue in human kidney diseases.