Synthesis and characterization of a novel thermoresponsive copolymer series and their application in cell and cell sheet regeneration |
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| Authors: | Maria E. Nash William M. Carroll Diego Velasco Javier Gomez Alexander V. Gorelov Dmitry Elezov |
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| Affiliation: | 1. School of Chemistry, National University of Ireland Galway , Galway , Ireland;2. Department of Applied Macromolecular Chemistry , Institute of Polymer Science and Technology CSIC , Juan de la Cierva 3, Madrid , Spain;3. School of Chemistry, National University of Ireland Galway , Galway , Ireland;4. Department of Agrochemistry and Environment , Instituto de Biología Molecular y Celular Universidad Miguel Hernández de Elche , c/ Avenida de la Universidad s/n. 03202, Elche-Alicante , Spain;5. School of Chemistry and Chemical Biology, University College Dublin , Dublin , Ireland;6. National Centre for Biomedical Engineering Science, National University of Ireland , Galway , Ireland |
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| Abstract: | A series of poly-(N-isopropyl acrylamide)-based copolymers were developed with a view to biomedical applications, specifically cell cultivation and recovery. Ethylpyrrolidone methacrylate (EPM), the monomer of poly-(ethylpyrrolidone methacrylate) (pEPM), which is itself thermoresponsive, was copolymerized with N-isopropylacrylamide in varying ratios to create this novel thermoresponsive copolymer series. Characterization indicated a moderate increase of copolymer lower critical solution temperature with increasing EPM content. Films of the copolymers successfully hosted cells to monolayer. Cells detached from the copolymers upon temperature reduction with cell to cell junctions maintained, avoiding the damage which can be caused using conventional detachment techniques. These results indicate that these copolymers are highly cell compatible and may be useful for a range of biomedical applications. |
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| Keywords: | cell culture cell detachment radical polymerization spin coating thermoresponsive |
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