Effects of latex membrane on guided regeneration of long bones |
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Authors: | Bruna Leonel Carlos Jéssica Suzuki Yamanaka Gabriela Rezende Yanagihara Ana Paula Macedo Plauto Christopher Aranha Watanabe João Paulo Mardegan Issa |
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Affiliation: | 1. Department of Biomechanics, Medicine and Locomotor Apparatus Rehabilitation, Ribeir?o Preto Medical School, University of S?o Paulo, Ribeir?o Preto, Brazil;2. Department of Dental Materials and Prosthesis, Dental School of Ribeir?o Preto, University of S?o Paulo, Ribeir?o Preto, Brazil;3. Department of Stomatoligy, Collective Health and Legal Dentistry, Dental School of Ribeir?o Preto, University of S?o Paulo, Ribeir?o Preto, Brazil;4. Department of Basic and Oral Biology, Dental School of Ribeir?o Preto, University of S?o Paulo, Ribeir?o Preto, Brazil |
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Abstract: | AbstractNatural latex extracted from Hevea brasiliensis is one of the materials pointed out as potential tissue regenerators. The use of latex-based membranes in bone regeneration might be an alternative to stimulate bone formation. The aim of this study was to evaluate the effects of latex membranes in guided bone regeneration of defects produced in long bones of rats. Sixty rats were equally divided into latex and control groups, and each group was subdivided into two subgroups according to treatment duration of 1 and 4 weeks. Bone defects with 2.5?mm in diameter were surgically made in the left tibia. In the animals of the latex group, a latex membrane was placed over the bone defect. The samples underwent quantitative histological analysis of bone formation and collagen matrix, immunohistochemical analysis of osteogenic protein markers, assessment of bone mechanical properties and bone densitometry, and radiological assessment. The osteocalcin immunostaining data were submitted to the generalized linear model test with two independent factors. For the other data, the multivariate ANOVA with two independent factors was performed. The use of the latex membrane significantly improved (p?0.005) the volume of newly formed bone, collagen type I matrix, expression of osteopontin, and bone stiffness, both in the early and late stages of regeneration. In conclusion, the latex membrane was able to promote bone regeneration in long bones. |
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Keywords: | Latex Hevea brasiliensis guided bone regeneration stereology |
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