Characterization of the release profile of doxycycline by PLGA microspheres adjunct to non-surgical periodontal therapy |
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Authors: | Lucas Alves Moura Fernanda Vieira Ribeiro Talita Bianchi Aiello Eliana Ap De Rezende Duek Enilson Antonio Sallum Francisco Humberto Nociti Junior |
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Institution: | 1. Division of Periodontics, Department of Prosthodontics and Periodontics, Piracicaba Dental School, State University of Campinas (UNICAMP), Av. Limeira 901, Caixa Postal: 052, CEP: 13414-903 Piracicaba, S?o Paulo, Brazil;2. Laboratory of Biomaterials, Pontifical Catholic University of S?o Paulo, Sorocaba, Brazil;3. Department of Periodontics, Paulista University, S?o Paulo, Brazil;4. Laboratory of Biomaterials, Pontifical Catholic University of S?o Paulo, Sorocaba, Brazil |
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Abstract: | The aim of this pilot study was to assess the release of locally delivered doxycycline by poly (l-lactide-co-glycolide) (PLGA) microspheres in the periodontal pocket of patients with chronic periodontitis, treated by non-surgical periodontal therapy. Nineteen sites of non-adjacent teeth of four different patients were evaluated. Five milligram of PLGA microspheres loaded with 16 doxycycline hyclate (DOX) was administered per periodontal site. To quantify DOX released into the periodontal pocket, gingival crevicular fluid (GCF) was collected from the sites on days 2, 5, 7, 10, 15, and 20 after DOX application, and high-performance liquid chromatography was performed. Data were statistically assessed by ANOVA/Tukey test. At days 2, 5, and 7, the DOX concentration was stably sustained (23.33 ± 1.38, 23.4 ± 1.82, and 22.75 ± 1.33 μg/mL, respectively), with no significant differences over these assessment times (p > 0.05). At days 10 and 15, a tendency was observed toward a decrease in DOX concentration (21.74 ± 0.91 and 20.53 ± 4.88 μg/mL, respectively), but a significant decrease in GCF drug concentration (19.69 ± 4.70 μg/mL) was observed only on day 20. The DOX delivery system developed demonstrated a successful sustained release after local administration, as an adjunct to non-surgical periodontal therapy. |
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Keywords: | HPLC delivery system microspheres doxycycline PLGA FTIR non-surgical periodontal therapy |
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