妊娠高血压综合征孕妇血清SOD、GSH-px活性和脂质过氧化水平的变化

①目的　探讨妊娠高血压综合征 (妊高征 )病人外周静脉血血清中超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH px)活性以及脂质过氧化终产物丙二醛 (MDA)含量的变化及其与妊高征发生、发展的关系。 ②方法　用酶生化法和硫代巴比妥酸法检测 2 7例非妊娠妇女、32例正常妊娠妇女和 5 1例妊高征孕妇血清中SOD、GSH px活性以及MDA含量。 ③结果　正常妊娠组SOD、GSH px活性及MDA含量较非妊娠组明显增高 (F =3.2 6～ 11.4 8,q =2 .6 9～ 8.2 7,P <0 .0 1) ,SOD/MDA、GSH px/MDA比值差异无显著性 (P >0 .0 5 )。妊高征组MDA含量显著高于正常妊娠组 (q =4 .34,P <0 .0 1) ,且病情越重 ,增加越明显 (q =4 .78～ 8.2 6 ,P <0 .0 1) ;而SOD、GSH px活性、SOD/MDA、GSH px/MDA显著低于正常妊娠组 ,差异有显著性 (q =2 .0 1～ 9.37,P <0 .0 1)。④结论　妊高征的发生、发展与机体氧化和抗氧化平衡失调有关 ,SOD/MDA、GSH px/MDA可作为早期判定妊高征病情的指标。

CHANGES OF SOD,GSH-px AND MDA IN PATIENTS WITH PREGNANCY-INDUCED HYPERTENSION

Objective To study the changes of superoxide dismutase(SOD), glutathione peroxidase (GSH-px) and malondialdehyde (MDA) in peripheral venous blood of patients with pregnancy-induced hypertension syndrome(PIH). Methods The activity of SOD, GSH-px and content of MDA in the serum of peripheral blood of non-pregnant women, normal pregnant women and PIH women were measured by enzyme-biochemical tecnique. Results The activity of SOD and GSH-px and MDA content in the normal pregnant group were higher than those in the non-pregnant group (F=3.26-11.48, q =2.69- 8.27, P< 0.01) , while the difference of SOD/MDA and GSH-px/MDA between these two groups was not significant (F=1.21, 1.32; q=0.58, 0.62; P> 0.05). MDA content was much higher in the PIH group than that in the normal pregnant group (q=4.34, P<0.01). The activity of SOD and GSH-px and the ratios of SOD/MDA and GSH-px/MDA were markedly lower in the PIH group, as compared with those of the normal pregnant group, the differences being significant (q=2.01-9.37, P<0.01 ). Conclusion The pathogenesis and the development of PIH may be associated with the imbalance of oxidation and antioxidation.