放射性视网膜病变大鼠玻璃体与血清血管内皮生长因子的相关研究

目的通过放射性^60Co照射SD大鼠制作放射性视网膜病变（RR）模型,探讨在RR的发展中,血管内皮生长因子（VEGF）的作用。方法45只SD大鼠,随机分为正常对照组、10Gy组和30Gy组,每组15只。^60Co射线照射制作RR模型。分别于3、6、9个月时检测RR大鼠血清及玻璃体中的VEGF质量浓度,并制作大鼠视网膜毛细血管铺片。结果30Gy组与10Gy组大鼠玻璃体中VEGF浓度较正常对照组增高,差异均有统计学意义（P〈0.01）。30Gy组与10Gy组大鼠血清中VEGF质量浓度与正常对照组比较差异均无统计学意义（P〉0.05）。实验组大鼠视网膜毛细血管铺片结果显示,随时间延长视网膜微血管病变逐渐加重。结论在RR大鼠模型中,VEGF参与RR的发展。随时间延长玻璃体中VEGF质量浓度增加,后者与RR视网膜微血管病变的发展呈正相关,为临床上治疗RR提供理论依据。

Research on vascular endothelial growth factor in vitreous and serum in radiation retinopathy rat

Objective Radiation retinopathy is a serious complication following the radiation irradiation of ocular tumour. Radiation retinopathy is mainly characterized by neovascularization of retina. The goal of this study was to observe the content of vascular endothelial growth factor （VEGF） in serum and vitreous of Spregue-Dawley （SD） rats with radiation retinopathy rats and explore the role of VEGF in the development of radiation retinopathy. Methods dioative-ray was used to irradiate 30 male SD rats with the absorbing dose of 0.8/min. The irradiated rats were randomly divided into 10 Gy irradiation group （12.5 min）and 30 Gy irradiation group（37.5 min）. Other normal 15 SD rats were as control group. The rats were sacrificed and serum and vitreous specimens were collected in 3,6 and 9 months after irradiation. Retina was denuded for trypsin digestion and stretched preparation. VEGF contents in serum and vitreous were detected by ELISA. Results In 30 Gy irradiation group, the VEGF concentrations in vitreous was （84.66±5.97）ng/mL,（316.27±9.62） ng/mL and （663.98 ±5.93） ng/mL in 3,6 and 9 months after irradiation, being higher than that of normal control group （77.04±4.36） ng/mL （ P 〈 0. 01 ）. The VEGF concentration in serum was （5.73±2.65 ） ng/mL, （7.09±0.85 ） ng/mL and （7. 16±2. 61 ） ng/mL in 3,6 and 9 months respectively,showing a insignificantly difference in comparison with control group（6. 78±3.61 ） ng/mL （ P 〉 0. 05 ）. In 10 Gy irradiation rats,concentration of VEGF in vitreous was （72. 32±2. 16） ng/mL, （75.06±5.55 ） ng/mL and （85.05±4. 46） ng/mL in 3,6 and 9 months respectively and that of normal control group was （70. 27 ±1.65） ng/mL,showing a significant difference among them （P 〈 0. 01 ）. The VEGF concentration of serum in 10 Gy irradiation group was （4. 85 ± 3. 16） ng/mL, （6. 29 ±2. 40） ng/mL and （5. 11 ±2.67） ng/mL in 3,6 and 9 months respectively,and that of the control group was （5.98±3.64） ng/mL