| 一种小鼠胸腺基质细胞和活化胸腺细胞共表达分子的研究 |
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| 引用本文： | 陈慰峰,何其华,肖士云. 一种小鼠胸腺基质细胞和活化胸腺细胞共表达分子的研究[J]. 中国免疫学杂志, 2000, 16(9): 459-461 |
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| 作者姓名： | 陈慰峰 何其华 肖士云 |
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| 作者单位： | 1. 北京医科大学免疫系T细胞研究室，北京 100083
2. 江西省赣南医学院免疫学教研室，赣州 341000 |
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| 基金项目： | 本课题受国家自然科学基金资助(No．39230320) |
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| 摘 要： | 目的：研究抗小鼠胸腺基质细胞（ＭＴＳＣ）Ｐｆ１８－３单抗识别分子的表达特性。方法：采用流式细胞（ＦＡＣＳ）和激光扫描共焦显微镜（ＣＬＳＭ）分析ＴＳＣ单抗Ｐｆ１８－３染色特征。结果 ：ＦＡＣＳ分析发现Ｐｆ１８－３单抗识别分子可表达于胸腺、胎肝和骨髓等不同来源的基质细胞系和腹腔巨噬细胞表面。新鲜分离的胸腺细胞Ｐｆ１８－３识别分子表达阴性，但ＣｏｎＡ活化可诱导并上调其表达，随活化时间延长表达增高，且主要
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| 关 键 词： | 单克隆抗体 FACS CLSM T细胞 胸腺基质细胞 |
Studies on the characterization of a molecule expressed on murine thymic stromalcells and activated thymocytes |
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| XIAO Shi-Yun,CHEN Wei-feng,HE Qi-Hua. Studies on the characterization of a molecule expressed on murine thymic stromalcells and activated thymocytes[J]. Chinese Journal of Immunology, 2000, 16(9): 459-461 |
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| Authors: | XIAO Shi-Yun CHEN Wei-feng HE Qi-Hua |
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| Abstract: | Objective: To identify the expression of the molecule recognized by Pf18 3 mAb (Pf18 3 molecule) on various cells Methods: The expression of Pf18 3 molecule was assayed by flow cytometry and confocal laser scanning microscope Results: The molecule recognized by Pf18 3 mAb expressed on TSC and other stromal cells Whereas, fresh thymocytes were Pf18 3 negative Interestingly, the expression of Pf18 3 molecule was gradually up regulated on thymocytes after activation by ConA This molecule mainly expressed on CD4 CD8 and CD4 CD8 - cells Under confocal laser scanning microscope, the staining of fluorescence showed as ring around the cell,it changed gradually stronger and thicker with activation Conclusion: This study indicated that the Pf18 3 molecule was co expressive molecule of MTSC and activated thymocytes,it was concerned closely about the activation of CD4 CD8 and CD4 CD8 - cells |
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| Keywords: | Monoclonal antibody Cell FACS CLSM |
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