Proteomic Analysis of Chronic Restraint Stress-Induced Gan (肝)-Stagnancy Syndrome in Rats |
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引用本文: | 孙学刚,钟小兰,刘志锋,蔡红兵,范钦,王启瑞,刘强,宋雨鸿,贺松其,张绪富,吕志平.Proteomic Analysis of Chronic Restraint Stress-Induced Gan (肝)-Stagnancy Syndrome in Rats[J].Chinese Journal of Integrated Traditional and Western Medicine,2010,16(6):510-517. |
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作者姓名: | 孙学刚 钟小兰 刘志锋 蔡红兵 范钦 王启瑞 刘强 宋雨鸿 贺松其 张绪富 吕志平 |
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作者单位: | [1]Laboratory of Traditional Chinese Medicine Syndrome, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou (510515), China [2]People's Hospital of Huadu, Guangzhou (510800), China [3]Guangzhou General Hospital, Guangzhou Military Region PLA, Guangzhou (510010), China |
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基金项目: | Supported by the National Natural Science Foundation of China (No. 30371713, 30672769) |
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摘 要: | Objective: To analyze the proteomic characteristics of Gan (肝))--ssttaaggnnaannccyy syndrome ((GGSSSS)) by seeking the differential protein in blood and tissues of GSS model rats.Methods: GSS model rats were established by chronic restraint stress,keeping rats in restrain chamber for 6 h every day for 21 successive days.Their blood and liver samples were collected at the end of experiment for differential protein detection with methods of isoelectrofocusing and polyacrylamide SDS-PAGE,silver staining,and scanning.The gel images were analyzed with Imagemaster 2D Elite software,and the excavated differential protein spots were identified with matrix assistant laser resolving TOF mass spectrometry,Western blot,ELISA,and RT-PCR,respectively.Results: A method for isolating the protein in blood serum and tissues by two-dimensional gel electrophoresis was established and optimized.Six serum proteins and three liver proteins that differentially expressed were identified.The down-regulated differential proteins in serum of GSS model rats were serum albumin precursor,beta 1 globin,antibody against muscle acetylcholine receptor,Ig lambda-2 C region,and transthyretin (TTR),and those in liver tissue were aryl sulfotransferase,enoyl-CoA hydratase,and TTR.TTR down-regulation was found in both serum and liver.Preliminary biological information analysis showed that these differential proteins involved in immune,neuroendocrine,nutrition,and substance metabolism.Conclusion: Proteomic analysis of differential proteins showed that TTR,aryl sulfotransferase,and enoyl-CoA hydratase expressions are downregulated in the GSS model rats,suggesting that the susceptibility of cancer could be enhanced by chronic stress.
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关 键 词: | 蛋白质组学 大鼠模型 应激性 综合征 慢性 血清蛋白 学分 停滞 |
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