Molecular Cytogenetic Characterization in Four Pediatric Pheochromocytomas and Paragangliomas |
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Authors: | Ales Vicha Milena Holzerova Anna Krepelova Zdenek Musil Pavel Prochazka David Sumerauer Roman Kodet Tomas Eckschlager Marie Jarosova |
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Institution: | 1.Department of Pediatric Hematology and Oncology, 2nd Medical School,Charles University and University Hospital Motol,Prague 5- Motol,Czech Republic;2.Department of Hemato-oncology, Medical School,Palacky University and University Hospital,Olomouc,Czech Republic;3.Institute of Biology and Medical Genetics, 2nd Medical School,Charles University and University Hospital Motol,Prague,Czech Republic;4.Department of Pathology and molecular medicine, 2nd Medical School,Charles University and University Hospital Motol,Prague,Czech Republic;5.Institute of Biology and Medical Genetics,Charles University 1st Faculty of Medicine and General Teaching Hospital,Prague,Czech Republic;6.Department of Antropology and Human genetics,Charles University Faculty of Science,Prague,Czech Republic |
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Abstract: | Pheochromocytomas (PCCs) are rare tumors among children and adolescents and therefore are not genetically well characterized.
The most frequently observed chromosomal changes in PCC are losses of 1p, 3q and/or 3p, 6q, 17p, 11q, 22q, and gains of 9q
and 17q. Aberrations involving chromosome 11 are more common in malignant tumors. Unfortunately information about gene aberrations
in childhood PCC’s is limited. We used comparative genomic hybridization (CGH) and array comparative genomic hybridization
(aCGH) to screen for copy number changes in four children suffering from pheochromocytoma or paraganglioma. Patients were
diagnosed at the age 13 or 14 years. Bilateral pheochromocytoma was associated with von Hippel-Lindau syndrome (VHL). Multiple
paraganglioma was associated with a germline mutation in SDHB. We found very good concordance between the results of CGH and
aCGH techniques. Losses were observed more frequently than gains. All cases had a loss of chromosome 11 or 11p. Other aberrations
were loss of chromosome 3 and 11 in sporadic pheochromocytoma, and loss of 3p and 11p in pheochromocytoma, which carried the
VHL mutation. The deletion of chromosome 1p and other changes were observed in paragangliomas. We conclude that both array
CGH and CGH analysis identified similar chromosomal regions involved in tumorigenesis of pheochromocytoma and paragangliomas,
but we found 3 discrepancies between the methods. We didn’t find any, of the proposed, molecular markers of malignancy in
our benign cases and therefore we speculate that molecular cytogenetic examination may be helpful in separating benign and
malignant forms in the future. |
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