| 人晶体上皮细胞的体外培养 |
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| 引用本文： | 郑健梁 彭莹. 人晶体上皮细胞的体外培养[J]. 眼科学报, 1997, 13(4): 170-172 |
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| 作者姓名： | 郑健梁 彭莹 |
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| 作者单位： | 中山医科大学中山眼科中心，中山医科大学中山眼科中心，衡阳医学院附属第一医院眼科 广州 510060，广州 510060，衡阳 421001 |
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| 摘 要： | 目的:提供一种简单可行,易于掌握的体外培养人晶体上皮细胞的方法。方法:用无齿显微镊子将晶体囊膜分离出来,剪碎后直接移至细胞培养瓶中培养,当细胞长出融合后,用胰蛋白酶消化传代。结果:接种培养4天后,可见晶体上皮细胞开始长出,并以贴壁方式生长。结论:用此方法体外培养人晶体上皮细胞,具有简单、快捷、成功率高的优点。眼科学报1997;13:170—172。
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| 关 键 词： | 白内障 晶体上皮细胞 细胞培养 |
Human Lens Epithelial Cells Culture in Vitro |
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| Jianliang Zheng,Yizhi Liu,Ying PengZhongshan Ophthalmic Center,Sun Yat-sen University of Medical Sciences,Guangzhou ,China. Human Lens Epithelial Cells Culture in Vitro[J]. Eye science, 1997, 13(4): 170-172 |
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| Authors: | Jianliang Zheng Yizhi Liu Ying PengZhongshan Ophthalmic Center Sun Yat-sen University of Medical Sciences Guangzhou China |
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| Affiliation: | Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China. |
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| Abstract: | PURPOSE: To provide a simple and rapid procedure to culture human len epithelial cells in vitro. METHODS: Specimens of capsule lentils were isolated from lens by microscissors. They were cut into small segments and then directly implanted into basks(culture bottles). The tissue pieces were cultured in a incubation at 37 degrees C with PR1640 until attached cells reached confluence. The len epithelium were passaged by tropism. RESULTS: Len epithelial cells usually appeared after 4 days in incubation. The cells growed rapidly in a way of attachments. CONCLUSION: Len epithelial cells were easily cultured with our method. The procedure is simple, rapid and highly successful. |
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| Keywords: | cataract Len epithelial cell cell culture |
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