γ—干扰素及肝素抑制兔晶体上皮细胞生长的实验研究

目的:研究γ—干扰素及肝素对体外培养的兔晶体上皮细胞(RLEC)的生长抑制作用,探讨后发性白内障的防治方法。方法:RLEC原代细胞来自正常健康的新西兰大白兔。胰酶消化法传代。第三代细胞分别与0.1～10000u/ml剂量的γ—干扰素及100～5000u/ml的肝素孵育48小时,用MTT法检测γ—干扰素及肝素对晶体上皮细胞生长的抑制作用效果。结果:0.1～100u/ml剂量组的γ—干扰素对体外培养的RLEC无明显的抑制作用,1000u/ml及10000u/ml剂量组的γ—干扰素则对RLEC有较明显的抑制作用,其增殖抑制率分别为27％及38％。100～5000u/ml剂量组的肝素对RLEC均有明显的抑制作用。结论:一定浓度的γ—干扰素及肝素对兔晶体上皮细胞生长具有直接抑制作用。眼科学报1997;13:167—169。

Study of antiproliferative effect of gamma-interferon and heparin on rabbit lens epithelial cell (RLEC) cultured in vitro]

PURPOSE: To study the antiproliferative effect of gamma-interferon and heparin on rabbit lens epithelial cell(RLEC). METHODS: First passage RLEC was obtained from healthy New zealand white rabbits. Third passage cells were incubated with gamma-interferon with concentration from 0.1 u/ml to 10,000 u/ml and heparin with concentration from 100-5,000 u/ml for 48 hours. The Mosmann's MTT colorimeltric assay was used to quantitate the RLEC proliferative activation. RESULTS: In lower concentration groups (0.1-100 u/ml), gamma-interferon had no antiproliferative effect on RLEC, while in higher concentration groups (1,000 u/ml-10,000 u/ml) it exhibited some antiproliferative effect on RLEC, the rate of inhibition were 27% and 38%, respectively. Heparin of all experimental groups had antiproliferative effect on RLEC. CONCLUSION: gamma-interferon and heparin with certain concentration can inhibit proliferation of RLEC directly.