日本血吸虫感染过程中糖酵解途径对调节性T细胞产生的影响

目的 探索日本血吸虫感染过程中糖酵解途径对小鼠调节性T（Treg）细胞数量和功能的影响。方法 建立日本血吸虫感染小鼠模型，用糖酵解抑制剂2?Deoxy?D?glucose（2DG）或PBS对日本血吸虫感染小鼠进行6次腹腔注射后，分离脾脏细胞和肠系膜淋巴结，采用流式细胞术（FCM）检测分离得到的细胞中Glut1+CD4+ T细胞以及Treg细胞比例。结果 未感染组小鼠脾脏（43.58%±2.50% vs. 21.15%±0.96%；t = 8.834，P < 0.01）和肠系膜淋巴结中Glut1+CD4+ T细胞比例（38.97%±1.97% vs. 28.40%±2.11%；t = 3.662，P < 0.05）均显著高于感染日本血吸虫8周小鼠，但未感染组小鼠脾脏（6.83%±0.21% vs. 13.30%±0.35%；t = 15.65，P < 0.01）和肠系膜淋巴结中Treg细胞比例（8.26%±0.15% vs. 14.37%±0.44%；t = 13.14，P < 0.01）均显著低于感染组小鼠。感染小鼠给与2DG腹腔注射后，脾脏（15.50%± 0.76% vs. 13.07%±0.15%；t = 3.130，P < 0.05）和肠系膜淋巴结中Treg细胞比例（17.00%±0.41% vs. 13.83%±0.18%；t = 6.947，P < 0.01）显著高于给与PBS注射小鼠。结论 糖酵解途径抑制了日本血吸虫感染小鼠Treg细胞分化。

Effects of glycolytic pathway on generation of regulatory T cells during Schistosoma japonicum infection

Objective To assess the influence of glycolytic pathway on the proportion and numbers of regulatory T cells during Schistosoma japonicum infection. Methods A S. japonicum?infected mouse model was established， and C57/BL6 male mice infected with S. japonicum were subjected to intraperitoneal injections of with the glycolytic inhibitor 2?Deoxy?D?glucose （2DG） or PBS for 6 times， and then the cells from spleen or mesenteric lymph nodes （LNs） were isolated and analyzed by flow cytometry （FCM） to detect the percentage of Glut1+CD4+ T cells and Treg cells. Results The proportions of Glut1+CD4+ T cells in the spleen （43.58%±2.50% vs. 21.15%±0.96%； t = 8.834， P < 0.01） and mesenteric LNs （38.97%±1.97% vs. 28.40%±2.11%； t = 3.662， P < 0.05） were higher in the normal mice than those in the infected mice， and the percentages of Treg cells in the spleen （6.83%±0.21% vs. 13.30%±0.35%； t = 15.65， P < 0.01） and LNs （8.26%±0.15% vs. 14.37%±0.44%； t = 13.14， P < 0.01） were lower in the normal mice than those in the infected mice. In addition， the proportions of Treg cells in the spleen （15.50%±0.76% vs. 13.07%±0.15%； t = 3.130， P < 0.05） and LNs （17.00% ± 0.41% vs. 13.83% ± 0.18%； t = 6.947， P < 0.01） were higher in the infected mice injected intraperitoneally with 2DG than those in the infected mice injected intraperitoneally with PBS. Conclusion Glycolytic pathway inhibits Treg differentiation in the spleen and mesenteric LNs of S. japonicum?infected mice.