| 博尔纳病病毒荧光定量PCR试剂盒扩增缓冲液的优化 |
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| 引用本文： | 金戈,徐晓艳,张亮,马丽华,黄荣忠,邓婧,李文娟,房亮,谢鹏. 博尔纳病病毒荧光定量PCR试剂盒扩增缓冲液的优化[J]. 第三军医大学学报, 2011, 33(13): 1323-1327 |
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| 作者姓名： | 金戈 徐晓艳 张亮 马丽华 黄荣忠 邓婧 李文娟 房亮 谢鹏 |
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| 作者单位： | 1. 400016,重庆,重庆医科大学:附属第一医院神经内科;400016,重庆,重庆医科大学:神经科学研究中心,重庆市神经生物学重点实验室
2. 重庆医科大学:神经科学研究中心,重庆市神经生物学重点实验室,重庆,400016 |
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| 基金项目： | 国家重点基础研究发展计划(973计划,2009CB918302); 重庆市自然科学基金(CSTC2010BB5393); 重庆市卫生局医学科学技术研究项目(2010-2-102)~~ |
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| 摘 要： | 目的 选择并优化博尔纳病病毒荧光定量PCR TaqMan探针法反应体系中的扩增缓冲液,使PCR反应体系对该病毒p24模板基因的扩增效率得到明显提高.方法 通过常规PCR比较选择3种常用酸(盐酸、磷酸和硫酸)所滴定的缓冲液体系,在其基础上选择适合浓度的硫酸铵配制TSS buffer,在TSS buffer中分别加入四甲基...
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| 关 键 词： | PCR扩增缓冲液 PCR试剂盒 博尔纳病病毒 |
Optimization of PCR buffer for Borna disease virus real-time PCR diagnostic kit |
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| Jin Ge,Xu Xiaoyan,Zhang Liang,Ma Lihua,Huang Rongzhong,Deng Jing,Li Wenjuan,Fang Liang,Xie Peng. Optimization of PCR buffer for Borna disease virus real-time PCR diagnostic kit[J]. Acta Academiae Medicinae Militaris Tertiae, 2011, 33(13): 1323-1327 |
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| Authors: | Jin Ge Xu Xiaoyan Zhang Liang Ma Lihua Huang Rongzhong Deng Jing Li Wenjuan Fang Liang Xie Peng |
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| Affiliation: | Jin Ge1,2,Xu Xiaoyan1,Zhang Liang1,Ma Lihua1,Huang Rongzhong1,Deng Jing1,Li Wenjuan1,Fang Liang2,Xie Peng1,2(1Department of Neurology,First Affiliated Hospital,2Neuroscience Center,Key Laboratory of Neurobiology of Chongqing,Chongqing Medical University,Chongqing,400016,China) |
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| Abstract: | Objective To select and optimize the PCR buffer for Borna disease virus real-time fluorescence quantitative PCR diagnostic kit in order to significantly improve PCR amplification efficiency to BDV p24 gene.Methods Conventional PCR was used to contrast the PCR buffers which titrated with hydrochloric acid,phosphoric acid and sulfuric acid respectively,and the appropriate concentration of ammonium sulfate was further explored to prepare TSS buffer.Four PCR additives(TMAC,Triton X-100,DMSO and betaine) were ad... |
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| Keywords: | PCR buffer PCR kit Borna disease virus |
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