| PDGF对体外培养的中脑黑质DA能神经元的神经营养及保护作用 |
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| 引用本文： | 牛平,王耀山,吕永利,何祥.PDGF对体外培养的中脑黑质DA能神经元的神经营养及保护作用[J].解剖科学进展,2001,7(1):7-9. |
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| 作者姓名： | 牛平 王耀山 吕永利 何祥 |
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| 作者单位： | 中国人民解放军沈阳军区总医院神经内科 |
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| 基金项目： | 辽宁省自然科学基金资助项目! NO6 190 13 |
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| 摘 要： | 目的 探讨 PDGF对体外培养 DA能神经元的作用。方法 在培养孕 14～ 16 d大鼠胚胎黑质神经元中加入 PDGF,采用免疫组化并辅以计算机图像分析系统对培养细胞进行观察。结果 PDGF在培养早期具有促进中脑 DA神经元发育、长突起神经元突起延长和提高神经元存活率作用。结论 PDGF对体外培养 DA能神经元有营养和保护作用
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| 关 键 词： | 黑质 DA能神经元 血小板源性生长因子 |
| 文章编号： | 1006-2947(2001)01-7 |
| 修稿时间： | 2000年5月11日 |
The Nutrient and Protective Effects of Platelet-Derived Growth Factor (PDGF) on DA Neurons of the Mesencephalic Subtantia Nigra in Vitro |
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| Niu Ping,Wang Yaoshan,L Yongli,et al.The Nutrient and Protective Effects of Platelet-Derived Growth Factor (PDGF) on DA Neurons of the Mesencephalic Subtantia Nigra in Vitro[J].Progress of Anatomical Sciences,2001,7(1):7-9. |
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| Authors: | Niu Ping Wang Yaoshan L Yongli |
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| Abstract: | Objective To study the nutrient and protective effect of PDGF (Platelet-derived growth factor) on DA neurons of the mesencephalic subtantia nigra in vitro Methods Subtantia nigra neurons from rats E14-E16 were cocultured with PDGF and observed under the light microscopy by immunohistochemistry with computer image analysis (Luzex F real time image analysis). Results During all culture stage, PDGF promoted the development of mesencephalic DA neurons, increased numbers of alive cell and prolonged the length of long neurite-bearing neurons and had slight neurotrophic effect in the early stage. Conclusion PDGF has neutrient and protective effect on DA neurons of subtantia nigra in vitro. |
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| Keywords: | subtantia nigra dopaminergic neuron platelet-derived growth factor |
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