胃癌中医证型与胃癌转移相关基因E-cadherin的关系研究

目的:从基因蛋白表达上探索胃癌患者中医证的本质.方法:将收集到的术前胃癌患者病例资料按中医辨证分型标准确定其证型归属;用免疫组化EnVision二步法检测术后胃癌肿瘤标本中E-cadherin基因蛋白表达情况.结果:E-cadherin在100例胃癌患者中的阳性表达率为90%,证型间表达差异存在显著性(P<0.01);进一步两两比较示,痰湿凝结型、气血双亏型与脾胃虚寒型之间无明显差异,表达较高;瘀毒内阻型与肝胃不和型无明显差异,表达较低.结论:瘀毒内阻型与肝胃不和型胃癌患者的E-cadherin表达偏低,此两种证型肿瘤转移形成的途径可能与E-cadherin,即与肿瘤细胞间同质性黏附力降低相关.

Study on Relationship between TCM Syndrome Types of Gastric Carcinoma and Gastric Carcinoma Metastasis-Related Gene E-cadherin

Objective:To probe essence of TCM syndrome of gastric carcinoma from gene protein expression.Methods:Based on the collected data from pre-operative patients of gastric carcinoma,their TCM syndromes were determined by means of TCM syndrome classification criteria.E-cadherin gene protein expressions in tumor tissue after operation of gastric cancer were detected with EnVision two step immunohistochemical method.Results:The expression rate of E-cadherin was 90% in 100 cases of gastric carcinoma,with significant difference among different syndrome types(P<0.01).There was higher expression in the types of stagnation of phlegm-dampness,deficiency of both Qi and blood and deficiency-cold of the spleen and stomach,but with no significant difference among the 3 types;There was lower expression in the two types,stagnation of toxic substance in interior and incoordination of the liver and the stomach,with no significant difference between the two types.Conclusion:Metastasis of the two types,stagnation of toxic substance in interior and incoordination of the liver and the stomach,is possibly related with the lower expression of E-cadherin.