| 趋化因子CXCL10与肿瘤细胞RNA修饰树突状细胞抗前列腺癌研究 |
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| 引用本文： | 李博,赵庆利,伦晓勤,魏学斌,李顺,李青
. 趋化因子CXCL10与肿瘤细胞RNA修饰树突状细胞抗前列腺癌研究[J]. 山东大学学报(医学版), 2009, 47(2): 10-13 |
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| 作者姓名： | 李博 赵庆利 伦晓勤 魏学斌 李顺 李青
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| 作者单位： | 山东大学附属千佛山医院泌尿外科,济南,2513014;济南医院职业病科,济南,250014 |
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| 基金项目： | 山东省自然科学基金，山东省医药卫生科研项目 |
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| 摘 要： | 目的探讨肿瘤细胞RNA和趋化因子CXCL10基因共同修饰的树突状细胞(DC)瘤苗对小鼠抗肿瘤免疫反应的诱导作用。方法将小鼠RM 1前列腺癌细胞RNA加载小鼠骨髓来源的DC后,通过脂质体转染法将CXCL10基因导入DC,制备前列腺癌瘤苗;体内外实验检测该瘤苗诱导的抗前列腺癌免疫治疗作用。结果转染CXCL10基因的DC上清对淋巴细胞有较强的趋化作用;DC瘤苗所诱导的特异性效应细胞(CTL)对RM 1细胞具有特异性杀伤作用;经DC瘤苗治疗的荷瘤小鼠模型瘤体生长减慢,存活期延长;该瘤苗还具有明显的免疫保护作用。结论构建的前列腺癌DC瘤苗能够有效诱导抗肿瘤免疫反应和免疫保护功能。
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| 关 键 词： | 树突细胞 RNA 肿瘤 CXCL10基因 前列腺肿瘤 小鼠 |
Immunotherapeutic effect of dendritic cell vaccine modified with the CXCL10gene and tumor cell RNA on mice with prostate cancer |
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| LI Bo,ZHAO Qing-li,LUN Xiao-qin,WEI Xue-bin,LI Shun,LI Qing. Immunotherapeutic effect of dendritic cell vaccine modified with the CXCL10gene and tumor cell RNA on mice with prostate cancer[J]. Journal of Shandong University:Health Sciences, 2009, 47(2): 10-13 |
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| Authors: | LI Bo ZHAO Qing-li LUN Xiao-qin WEI Xue-bin LI Shun LI Qing |
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| Affiliation: | 1.Department of Urology;Qianfoshan Hospital Affiliated to Shandong University;Jinan 250014;China;2.Department of Occupational Diseases;Jinan Hospital;China |
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| Abstract: | Objective To evaluate the effect of therapeutic vaccine against prostate cancer based on dendritic cell(DC) vaccine modified with tumor cell RNA and the CXCL10 gene both in vitro and in vivo.Methods Mouse bone marrow DC was pulsed with cell RNA from the RM-1 prostate cancer cell line and then transfected with the recombinant plasmid CXCL10/pcDNA3.1 by DOTAP liposome.The immunotherapeutic effects of DC vaccine on mice with prostate cancer were assessed.Results The DC vaccine increased the preferential chemot... |
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| Keywords: | Dendritic cells RNA neoplasm CXCL10 Genes Prostatic neoplasms Mice |
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