心房颤动患者氯电流相关通道CIC-3基因表达的研究

目的:研究心房颤动(AF)患者心房组织氯电流相关通道ClC-3的基因表达,探讨AF时心房组织ClC-3的mRNA表达改变及其意义.方法:将71例风湿性心瓣膜病接受换瓣手术患者分为3组:窦性心律组(SR组)31例,阵发性房颤组(PAF组)7例,持续性房颤组(CAF组)33例,术中获取右心耳组织,应用半定量逆转录-聚合酶链反应(RT-PCR)技术检测心房组织ClC-3的mRNA表达的相对含量.结果:与SR组相比,PAF组心房组织中ClC-3的mRNA表达增加,但无显著的统计学意义(P>0.05),CAF组的表达明显增加(P＜0.01),并与AF时程呈明显正相关(r=0.376,P=0.001).结论:房颤患者ClC-3的mRNA表达水平的增加可能是细胞容量调节的氯电流(ICl.VOL)上调的分子基础.

Study of chloride channel ClC-3 gene expression in patient with atrial fibrillation

AIM: To investigate alterations in gene expression of chloride channel ClC-3 in patients with atrial fibrillation (AF). METHODS: Right atrial appendages specimen were obtained from 33 patients with chronic AF(CAF group), 7 with paroxysmal AF(PAF group) and 31 matched controls in sinus rhythm (SR group) who underwent open-heart surgery. In this study, the semi-quantitative polymerase chain reaction (RT-PCR) was used to examine the mRNA expression of ClC-3 channel in atrial tissue. RESULTS: The content of ClC-3 mRNA in CAF group was higher than that in SR group(P< 0.01), and it was correlated with AF duration (r= 0.376,P= 0.001).There was no significant difference in the gene expression of ClC-3 between PAF group and SR group, although the content of ClC-3 mRNA was slightly higher than that in SR group (P> 0.05). CONCLUSION: The increased gene expression of chloride channel ClC-3 may be served as the molecular basis of I_ Cl.VOL remodeling in patients with atrial fibrillation (AF).