HPLC法同时测定没食子提取物中没食子酸、没食子酸甲酯和没食子酸乙酯的含量

目的建立高效液相色谱法测定没食子提取物中三种成分的含量方法。方法色谱柱:Waters Symmetry C18(4.6 mm×250 mm,5μm);流动相:甲醇-水(25∶75)(冰乙酸调p H至4.5);流速为1.0 m L·min-1,柱温:30℃,检测波长:273 nm。结果没食子酸、没食子酸甲酯、没食子酸乙酯的回收率分别为99.79%、99.60%、99.45%;线性范围分别为0.2³.2μg、0.0023.2μg、0.002⁰.16μg、0.010 80.16μg、0.010 8⁰.54μg。结论结果准确,重复性好,可用于该产品的质量控制。

SimultaNeous determiNatioN of gallic acid,metHyl gallate aNd etHyl gallate from gallNut extracts by HPLC

Objective To establish a method for the simultaneous determination of gallic acid,methyl gallate and ethyl gallate from gallnut extracts. MetHods HPLC was used to quantitative analysis. The Waters Symmetry C18（4. 6 mm × 250 mm,5 μm）was used,and mobile phase was composed of methanol-water（25：75）（adjust pH to 4. 5 with acetic acid）. The detection wavelength was 273 nm at 30℃. The flow rate was 1. 0 mL·min-1 . Results The gallic acid,methyl gallate and ethyl gallate＆#39;s spotting recovery were 99. 79%,99. 60%,99. 45%,and the linear ranges were 0. 2~3. 2 μg,0. 002~0. 16 μg,0. 010 8~0. 54 μg,respectively. CoNclusioN The results were accurate and the reproducibility was good. The method can be used for the quality control of the product.