可溶性Fas,可溶性Fas配体与细胞因子在1型糖尿病发病中的意义

目的　研究可溶性Fas(sFas)和可溶性Fas配体(sFasL)与细胞因子在1型糖尿病发病中的作用。方法　32名1型糖尿病患者和20名正常人的血清，采用夹心BAS-ELISA法分别检测sFas,sFasL,γ干扰素(IFN-γ)及白细胞介素-1(IL-1)含量。结果　1型糖尿病血清中sFas,IFN-γ及IL-1含量分别为(1　　546±685,1　　074±451与1　　406±721)ng/L，显著高于正常组；sFasL为(211±73)mg/L，低于正常组但差异无显著性。在1型糖尿病患者中高浓度sFas伴高IFN-γ者共12例，低浓度sFas伴低IFN-γ者共9例。结论　1型糖尿病患者血清中的sFas,IFN-γ及IL-1高于正常人，sFas与IFN-γ浓度呈正相关(r=0.79,P＜0.05)。对1型糖尿病患者血清进行sFas,IFN-γ及IL-1等检测可作为反映胰岛细胞病变的辅助指标，有助于对疾病的诊断与治疗。

The role of soluble Fas, soluble Fas ligand and cytokines in the pathogenesis of type 1 diabetes mellitus

Objective　To investigate the role of the concentration of soluble Fas (sFas), soluble Fas ligand (sFasL) and cytokines in the pathogenesis of type 1 diabetes mellitus (DM). Methods　The concentrations of sFas, sFasL, IFN-γ and IL-1 in sera isolated from 32 patients with type 1 DM and 20 healthy individuals were determined by the BAS-ELISA. Results　The concentrations of sFas, interferon-γ (IFN-γ) and interleukin-1 (IL-1) in sera from 32 patients with type 1 DM were (1　　546±685, 1 074±451 and 1 406±721)ng/L respectively, which were significantly higher than those of healthy individuals. Although the serum level of sFasL of type 1 DM patients was (211±73)ng/L which was lower than that of the healthy control but the difference was not significant. High concentrations of both sFas and IFN-γ were found in the sera of 12 patients with type 1 DM, while low concentrations of both sFas and IFN-γ were found in the sera of 9 patients with type 1 DM. Conclusion　The serum levels of sFas, IFN-γ and IL-1 of the patients with type 1 DM are significantly higher than those of healthy individuals. There　　is　　positive　　corralation　　between　　serum sFas and IFN-γ (r=0.79, P＜0.05). Analysing the concentrations of the sFas, IFN-γ and IL-1 in the sera from patients with type 1 diabetes mellitus may be helpful for evaluating the lesion of β cells of the patients with type 1 DM and useful for diagnosis and treatment.